Bone marrow mesenchymal stem cell transplantation for the treatment of temporomandibular joint osteoarthrosis

doi:10.3969/j.issn.2095-4344.2013.19.011

Abstract

Abstract:

BACKGROUND: With the development of stem cell technology, it is possible to repair cartilage injury using tissue engineering technology, and bone marrow mesenchymal stem cells have become the research focus because of their advantages.
OBJECTIVE: To observe the treatment effect of stem cells on rabbit temporomandibular joint disorders throughinjection of bone marrow mesenchymal stem cells cultured in vitro into a rabbit model of temporomandibular joint disorder.
METHODS: The bone marrow mesenchymal stem cells were cultured and identified with in vitro whole blood adherent method. Then the cells were amplified in vitro and induced to differentiate into chondrocytes for using. Intraarticular injection of type Ⅱ collagenase was performed to establish the animal model of temporomandibular joint disorder. In the experimental group, intraarticular injection of induced chondrocytes was performed; and in the control group, the rabbits were injected with un-induced cells. The treatment effect was compared between two groups through observing the chewing and tissue sections of the animals.
RESULTS AND CONCLUSION: A small amount of colony formation could be seen in the separated cells at 7-14 days; and at 20 days, the cells covered the bottom of the bottle basically. The stro-1⁺ and CD44⁺ flow cytometry and immunohistochemical determination showed the cells had the characteristics of stem cells; strong positive type Ⅱ collagen immunohistochemical staining of cells could be seen after induced into chondrocytes. Symptoms of unilateral chewing could be observed at 2 weeks after intraarticular injection of type Ⅱ collagenase. After intraarticular injection of bone marrow mesenchymal stem cells induced chondrocytes into the animal models, the unilateral chewing in the experimental group was weaker than that in the control group. Tissue sections showed that the induced chondrocytes could promote the repair of joint injury, and the cartilage and collagen generation in the experimental group was more than that in the control group. Intraarticular injection of bone marrow mesenchymal stem cells induced chondrocytes can promote the healing of rabbit temporomandibular joint osteoarthrosis.

Key words: stem cells, stem cell transplantation, chondroblast, induction and differentiation, temporomandibular joint disorder, issue engineering, treatment technology, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Yang Xing-hua, Peng Lin, Liu Yun-sheng, Xing Zai-chen, Li Hai-feng, Li Yu-qing, Liu Min. Bone marrow mesenchymal stem cell transplantation for the treatment of temporomandibular joint osteoarthrosis[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(19): 3488-3494.

Figures/Tables 5

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