Bone marrow mesencyhmal stem cell transplantation combined with edaravone for the treatment of cerebral infarction in rats

doi:10.3969/j.issn.2095-4344.2013.19.008

Abstract

Abstract:

BACKGROUND: Edaravone can scavenge free radicals and inhibit lipid peroxidation, which can improve microenvironment injury after central nervous system injury.
OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cell transplantation combined with edaravone on the treatment of cerebral infarction in rats.
METHODS: The models of cerebral infarction were made by middle cerebral artery occlusion. Model rats were randomly divided into control group, stem cell group (bone marrow mesenchymal stem cell transplantation) and combination group (edaravone+bone marrow mesenchymal stem cell transplantation). The concentration of 2.0×10⁶/mL bone marrow mesenchymal stem cell suspension or the concentration of 1 mL cell culture fluid was injected into the rat models through the caudal vein; at the same time, 3 mg/(kg•d) edaravone aqueous solution or normal saline was injected into the rat models through the abdominal cavity, for 5 days. The neurobehavioral scores were determined after 24 hours, 3 days and 1, 2 weeks after modeling. Aquaporin 9 and aquaporin-4 mRNA expressions were determined by reverse transcription-PCR. The rats were sacrificed for neuron and brain tissue around target morphological analysis by hematoxylin-eosin staining under a light microscopy.
RESULTS AND CONCLUSION: There was no difference in the neurological deficit scores among the three groups at 24 hour and 3 days after treatment (P > 0.05); while after 2 weeks, the neurological deficit score in the combination group was significantly lower than the stem cell group and control group (P < 0.05-0.01). In the stem cell group, the Aquaporin 9 and aquaporin-4 mRNA expressions were higher than the combination group but lower than the control group (P < 0.05). The number of CM-Dil-positive cells and neurons was higher in the combination group than the stem cell and control groups (P < 0.05). These findings indicate that transplanted bone marrow mesenchymal stem cells can survive and migrate to the peri-infarct region, and differentiate into neuron-like cells. Bone marrow mesencyhmal stem cell transplantation combined with edaravone can significantly improve neurological function of cerebral infarction rats.

Key words: stem cells, stem cell transplantation, edaravone, bone marrow mesenchymal stem cells, cell transplantation, cerebral infarction, neurological deficit score, aquaporin-4, aquaporin 9, stem cell photographs-containing paper

CLC Number:

R394.2

Sun Jing-jing, Zhang Peng, Zhou Guo-qing. Bone marrow mesencyhmal stem cell transplantation combined with edaravone for the treatment of cerebral infarction in rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(19): 3467-3473.

Figures/Tables 4

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