In vitro radiosensitivity of brain cancer stem cells in brain glioma

doi:10.3969/j.issn.2095-4344.2013.19.006

Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (19): 3455-3460.doi: 10.3969/j.issn.2095-4344.2013.19.006

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In vitro radiosensitivity of brain cancer stem cells in brain glioma

Zhao Zhong-fu^{1, 2}, Fan Rui-tai³, Yang Yong⁴, Sun Jian-rui², Hu Xiang⁵, Eileen Shen⁶, Yang Bo^{2, 7}

1 Department of Neurosurgery, Xuchang Central Hospital, Xuchang 461000, Henan Province, China
2 Department of Neurosurgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China
3 Radiotherapy Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China
4 Wei Erke Stem Cell Biotechnology (Liaoning) Co., Ltd., Benxi 117003, Liaoning Province, China
5 Shenzhen Beike Cell Engineering Institute, Shenzhen 518000, Guangdong Province, China
6 Dickinson College, Carlisle, PA 17013, U.S.A
7 Open Laboratory of Key Clinical Disciplines of Henan Province Higher Education, Zhengzhou 450052, Henan Province, China

Received:2012-09-16 Revised:2012-12-08 Online:2013-05-07 Published:2013-05-07
Contact: : Yang Bo, M.D., Professor, Doctoral supervisor, Department of Neurosurgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China; Open Laboratory of Key Clinical Disciplines of Henan Province Higher Education, Zhengzhou 450052, Henan Province, China yangbo96@126.com
About author:Zhao Zhong-fu★, Master, Chief physician, Department of Neurosurgery, Xuchang Central Hospital, Xuchang 461000, Henan Province, China; Department of Neurosurgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China zzf20041986@126.com Fan Rui-tai☆, M.D., Chief physician, Professor, Doctoral supervisor, Radiotherapy Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China Zhan Zhong-fu and Fan Rui-tai contributed equally to this paper.
Supported by:
Key Science and Technology Program of Henan Science and Technology Department, No. 112102310173*; Innovative Research Team-Building Foundation of the First Affiliated Hospital of Zhengzhou University, No. TD2011023*

Abstract

Abstract:

BACKGROUND: Radiotherapy effect on of brain glioma is not ideal, and there are various possible factors that influence the therapeutic effect.
OBJECTIVE: To investigate the in vitro radiosensitivity of brain cancer stem cells in brain glioma.
METHODS: Glioma cells were isolated and seeded into the serum-free medium containing growth factors for culture. Glioma cells from the most active cell site were subcultured to 3-5 generations. Glioma cell vitality was assessed to determine the optimal dose of X-ray radiation.
RESULTS AND CONCLUSION: Distinct difference in activities of proliferation could be found among cancer stem cells from different sites of glioma. X-ray dosages above 8 Gy had notable killing effect on brain cancer stem cells. Different irradiation dosage had different influence on cancer stem cells.

Key words: stem cells, tumor stem cells, brain cancer stem cells, glioma, growth factors, radiosensitivity, radiotherapy, irradiation, dose, individual positioning, proliferation activity, provincial grants-supported paper

CLC Number:

R394.2

Zhao Zhong-fu, Fan Rui-tai, Yang Yong, Sun Jian-rui, Hu Xiang, Eileen Shen, Yang Bo. In vitro radiosensitivity of brain cancer stem cells in brain glioma[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(19): 3455-3460.

Figures/Tables 4

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In vitro radiosensitivity of brain cancer stem cells in brain glioma

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