Bone marrow mesenchymal stem cells intervene maturation and differentiation of dendritic cells in aplastic anemia patients

doi:10.3969/j.issn.2095-4344.2013.19.004

Abstract

Abstract:

BACKGROUND: There are few reports about the influence of bone marrow mesenchymal stem cells on T cell proliferation in aplastic anemia patients in China. Besides, no study is reported in China on the topic that whether bone marrow mesenchymal stem cells affect the T cell proliferation in aplastic anemia patients by dendritic cell regulation. Therefore, its mechanism deserves further investigations.
OBJECTIVE: To explore effects of human bone marrow mesenchymal stem cells on dendritic cell immune regulation in patients with aplastic anemia.
METHODS: Human peripheral blood mononuclear cells-derived dendritic cells cultured for 5 days were co-cultured with human bone marrow mesenchymal stem cells at passage 3. Lipopolysaccharide and tumor necrosis factor were added to promote the maturation of dendritic cells. Flow cytometry was used to detect the expression of dendritic cell surface markers before and after coculture of bone marrow mesenchymal stem cells and immature and mature dendritic cells.
RESULTS AND CONCLUSION: Under the induction of lipopolysaccharide, prior to and following the coculture of immature dendritic cells and bone marrow mesenchymal stem cells, no changes of the expression of CD14, CD1a, CD83 and CD80 were observed (P > 0.05). Prior to and following the coculture of mature dendritic cells and bone marrow mesenchymal stem cells, the expression of CD14, CD1a, CD83 and CD80 was decreased (P < 0.05). Results suggested that bone marrow mesenchymal stem cells can inhibit the development and maturation of dendritic cells derived from monocytes of aplastic anemia patients, and exhibit immunoregulatory effects in patients with aplastic anemia.

Key words: stem cells, bone marrow-derived stem cells, anemia, aplastic, bone marrow, mesenchymal stem cells, coculture, mononuclear cells, dendritic cells, immunophenotype, inhibition, immunoregulation

CLC Number:

R394.2

Zhang Le-qin, Xiao Yang, Jiang Zu-jun, Xiao Hao-wen, Li Li, Pang Yan. Bone marrow mesenchymal stem cells intervene maturation and differentiation of dendritic cells in aplastic anemia patients[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(19): 3443-3448.

Figures/Tables 5

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