Insulin promotes the microvessel formation in fat grafts

doi:10.3969/j.issn.2095-4344.2013.18.013

Abstract

Abstract:

BACKGROUND: Currently, the in vitro cell culture experiment shows that insulin can not only promote the proliferation and repairing of vascular endothelia cells, but also can increase the proliferation and differentiation of preadipocytes. However, there are a few papers about the effect of insulin on revascularization in fat grafts through in vivo experiment.
OBJECTIVE: To investigate the influence of insulin on the microvessel formation in fat grafts of rats.
METHODS: Forty-eight Sprague Dawley female rats were used in this study, and then the rats were randomly divided into insulin group and control group. 1 mL fat graft was obtained from the side of fallopian tubes of the rats to make fat particles and then transplanted to the dorsal of the rats to establish the granule fat graft models. In the insulin group, granule fat grafts were pre-treated with insulin with the concentration of 300 mU/L before transplantation; the fat grafts in the control group without any treatment. The samples of fat grafts were harvested at 10, 20 and 28 days after the transplantation and treated with hematoxylin-eosin staining and CD34 vascular staining respectively. The changes of grafted adipocytes and the growth of microvessel were observed.
RESULTS AND CONCLUSION: Mature adipocytes could be observed in the hematoxylin-eosin staining sections, the size of adipocytes was smaller than that before transplantation, and part of the adipocytes were ruptured and shrinked. Hyperplasia of fibrous tissue was observed in two groups, and the number of fibrous tissue around the adipocytes in the insulin group was smaller than that in the control group. At 10 and 20 days after transplantation, the microvessel densities were (3.92±0.12) microvessels/high power field and (6.96±0.42) microvessels/high power field in the insulin group, (2.05±0.21) microvessels/high power field and (4.40±0.36) microvessels/high power field in the control group, respectively. Microvessel density in insulin group was higher than that in the control group, and there was a significant difference between two groups (P < 0.01). The result shows that insulin can improve the survival rate of autogenous fat grafts by promoting the microvessel formation.

Key words: organ transplantation, tissue transplantation, fat transplantation, adipocytes, insulin, microvessels, vascular endothelial cells, vascular anastomosis, survival rate, fibrous tissue, fibrosis, glucose metabolism, provincial grants-supported paper

CLC Number:

R318

Deng Ying, Zeng Ling-huan, Li Wei, Wu Yi. Insulin promotes the microvessel formation in fat grafts [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(18): 3318-3324.

Figures/Tables 2

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