Abstract:

BACKGROUND: Chinese herbal compounds mostly pose pharmacodynamic characteristics of “two-way adjustment” and “corrective bias” to restore the body’s balance. This experiment is proposed to verify the effect of Fuzi Lizhong Decoction and its disassembled prescription on deficiency of spleen-yang syndrome via warming yang and invigorating the spleen, further providing basic data for understanding the multi-node mechanism.
OBJECTIVE: To observe and evaluate the curative effects of Fuzi Lizhong Decoction and its disassembled prescription in the treatment of rats with spleen-yang deficiency syndrome under the conception of translational medicine.
METHODS: A model of deficiency of spleen-yang syndrome was made in Wistar rats through interscapular removal of brown fat and high fat diet under cold environment that changed every other day. After 70 days, the rat models were administered with Fuzi Lizhong Decoction, Lizhong Decoction and Fuzi Ganjiang Decoction, respectively. Another Wistar rats without modeling were used as controls that were fed with normal diet. Body mass, rectal temperature and area under rectal temperature curve were measured in all the groups.
RESULTS AND CONCLUSION: Body mass was increased slowest in the model group and fastest in the Fuzi Lizhong Decoction group (P < 0.05). The model group had lower rectal temperature than the other four groups and significantly differed from the control group in the rectal temperature (P < 0.05). There was a significant difference in the rectal temperature between the Fuzi Lizhong Decoction and model groups (P < 0.05). Area under rectal temperature curve in the model group was lower than that in the control group (P < 0.05). As compared with the models group, Fuzi Lizhong Decoction, Lizhong Decoction and Fuzi Ganjiang Decoction groups had the larger area under the rectal temperature curve (P < 0.05 or P < 0.01). Fuzi Lizhong Decoction, Lizhong Decoction and Fuzi Ganjiang Decoction can all improve the pathological conditions of rats with spleen-yang deficiency syndrome presenting with body mass reduction or slow growth and low temperature. Fuzi Lizhong Decoction has the best biologic effects among these three decoctions.

Key words: tissue construction, experimental modeling in tissue construction, Fuzi Lizhong Decoction, Lizhong Decoction, Fuzi Ganjiang Decoction, spleen-yang deficiency syndrome, disassembled prescription, model, animal, experimental study, traditional Chinese medicine, intervention, effect, comparison, provincial grants-supported paper