Establishing a nude mouse model of green fluorescence endometriosis

doi:10.3969/j.issn.2095-4344.2013.15.013

Abstract

Abstract:

BACKGROUND: It is simple to fabricate an animal model of endometriosis by subcutaneous injection of green fluorescence labeled endometrium of nude mice. Meanwhile, in vitro ectopic lesions can be observed dynamically within a certain period, which contributes to the depth study of endometriosis.
OBJECTIVE: To explore the method to establish an animal model of subcutaneous endometriosis in nude mice.
METHODS: Enhanced green fluorescent protein adenovirus transfected endometrium of nude mice was injected subcutaneously into six nude mice (fluorescent group) and non-transfected endometrium was injected into another six nude mice (control group). Green fluorescence intensity, duration of fluorescence labeled lesions, and histological changes of ectopic lesions in vivo were detected under stereological fluorescence microscope at 5, 10, 15, 20, 25, 30 days after injection.
RESULTS AND CONCLUSION: Five living fluorescence mouse models of endometriosis were induced successfully in the fluorescent group and another five living non-fluorescent nude mouse models of endometriosis were also prepared in the control group. There were 2-3 lesions per nude mouse. Development of ectopic lesions in the mice could be monitored under the stereological fluorescence microscope. The fluorescent area and intensity were decreased with time, which lasted for about 4 weeks. Fluorescence labeled lesions were invisible in the control group. Hematoxylin-eosin staining showed the typical endometrial glands and stroma structure. These findings indicate that this modified nude mouse model can enable the dynamic and quantitative observation of lesion growth and development without injury and contribute to the development of new drugs for endometriosis and the underlying mechanism of endometriosis．

Key words: tissue construction, experimental modeling in tissue construction, endometriosis, nude mice, green fluorescent protein, adenovirus, transfection, subcutaneously, animal models, stereological fluorescence microscope, other grants-supported paper

CLC Number:

R318

Yuan Hua, Gong Jian, Wang Jia-yuan, Huang Wei . Establishing a nude mouse model of green fluorescence endometriosis[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.15.013.

Figures/Tables 3

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