Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (5): 716-719.doi: 10.3969/j.issn.2095-4344.2431

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Establishment and evaluation of traumatic neuroma model

Wang Jing1, 2, Lu Changfeng2, Peng Jiang2, Zhu Chen3, Xu Wenjing2, Cheng Xiaoqing2, Fang Jie2, Zhu Yaqiong2, Zhao Yanxu2, Jiang Wen2, Xu Hongguang1, Wang Yu2   

  1. 1Department of Spinal Surgery, the First Affiliated Hospital of Wannan Medical College (Yijishan Hospital); 2Institute of Orthopaedics of PLA General Hospital, Beijing Key Laboratory of Regenerative Medicine, the Key Laboratory of National Military Warfare; 3the First Affiliated Hospital of University of Science and Technology of China
  • Received:2019-06-11 Revised:2019-06-12 Accepted:2019-07-20 Online:2020-02-18 Published:2020-01-09
  • Contact: Xu Hongguang, MD, Professor, Department of Spinal Surgery, the First Affiliated Hospital of Wannan Medical College (Yijishan Hospital), Wuhu 241001, Anhui Province, China Wang Yu, MD, Associace researcher, Institute of Orthopaedics of PLA General Hospital, Beijing Key Laboratory of Regenerative Medicine, the Key Laboratory of National Military Warfare, Beijing 100853, China
  • About author:Wang Jing, Master candidate, Department of Spinal Surgery, the First Affiliated Hospital of Wannan Medical College (Yijishan Hospital), Wuhu 241001, Anhui Province, China; Institute of Orthopaedics of PLA General Hospital, Beijing Key Laboratory of Regenerative Medicine, the Key Laboratory of National Military Warfare, Beijing 100853, China Lu Changfeng, MD candidate, Institute of Orthopaedics of PLA General Hospital, Beijing Key Laboratory of Regenerative Medicine, the Key Laboratory of National Military Warfare, Beijing 100853, China Wang Jiang and Lu Changfeng contributed equally to this work.
  • Supported by:
    the General Project of the Natural Science Foundation of Beijing, No. 7172202; the Youth Development Program of Military Medical Science and Technology, No. 16QNP144

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Abstract:

BACKGROUND: Traumatic neuroma is a common complication of peripheral nerve injury, and basic research on peripheral nerve repair and regeneration through a traumatic neuroma model is rarely reported.

OBJECTIVE: To establish and identify a traumatic neuroma model.

METHODS: The study was approved by the Laboratory Animal Ethical Committee of Chinese PLA General Hospital. Fifteen healthy Sprague-Dawley rats aged 8 weeks old were selected to expose the sciatic nerve under microscope, and the sciatic nerve was cut 1 cm away from the posterior gluteal muscle branch using microscissors. The proximal nerve was sutured to the surrounding muscle with 11-0 microlines, and the distal nerve was left open and reflexed. After 3 weeks, ultrasonic and histological examinations were used to verify the formation of neuroma.

RESULTS AND CONCLUSION: (1) Ultrasound: After 3 weeks of modeling, the proximal nerve showed obvious spindle enlargement, which was a hypoechoic parenchymal nodule without echo. (2) Gross observation: The proximal nerves were enlarged and slightly stiffened, with fibrous connective tissue hyperplasia and adhesion to the surrounding tissues. (3) Hematoxylin-eosin staining indicated hyperplasia of fibrous tissue and disorderly growth of nerve fibers in the tumor. Immunofluorescence staining (S100, NF200) revealed irregular hyperplasia of Schwann cells and nerve axons in the tumor. (4) These results suggest that the model of traumatic neuroma is successfully constructed.

Key words: peripheral nerves, peripheral nerve injury, nerve regeneration, neuroma, rats, animal models

CLC Number: