Restraint stress effects in type 1 diabetes rats

doi:10.3969/j.issn.2095-4344.2013.11.007

Abstract

Abstract:

BACKGROUND: A way to build psychological stress is restraint stress.
OBJECTIVE: To study the relationship of restraint stress and type 1 diabetes.
METHODS: Forty-eight male Sprague-Dawley rats underwent intraperitoneal injection of streptozotocin to rat models of type 1 diabetes mellitus. After the successful modeling, rats were randomly divided into two groups: stress group (the rats were exerted with restraint stress) and control group (no intervention). Six rats from either group were executed at 1, 2, 3 and 4 weeks after exerting stress, and the related stress markers were detected, including the levels of adrenal cortical hormone, corticosterone and glucagon in the serum. Fasting blood glucose was measured regularly in rats.
RESULTS AND CONCLUSION: In the stress group, the levels of glucose and glucagon, after a week of the stress, were higher than those in the control group (P < 0.05 or P < 0.001). Simultaneously, the levels of adrenal cortical hormone and corticosterone in serum were higher in the stress group compared with the control group (P < 0.05 or P < 0.001). These indicate that restraint stress can raise the blood sugar level of diabetic rats.

Key words: tissue construction, experimental modeling in tissue construction, restraint stress, type 1 diabetes mellitus, Sprague-Dawley rats, adrenal cortical hormone, corticosterone, other grants-supported paper

CLC Number:

R318

Wei Guang-xi, Wan Zhao-xia, Dai Hong-wei, Wang Mou. Restraint stress effects in type 1 diabetes rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(11): 1946-1950.

Figures/Tables 5

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