Effects of 1,25(OH)2D3 on microRNA-130b and
transforming growth factor beta 1 in renal tissue of rat models of diabetic
nephropathy

doi:10.3969/j.issn.2095-4344.1907

Abstract

Abstract:

BACKGROUND: 1,25(OH)₂D3 plays an important regulatory role in the development of diabetic nephropathy.

OBJECTIVE: To explore the effect of 1,25(OH)₂D3 on the expression of microRNA-130b and transforming growth factor β1 in kidney of diabetic nephropathy rats.

METHODS: The study was approved by the Laboratory Animal Ethical Committee of Laboratory Animal Center of Xinjiang Medical University. Twenty-five clean Sprague-Dawley rats were randomly divided into normal control group, diabetic nephropathy+1,25(OH)₂D3 group and diabetic nephropathy+peanut oil group. The latter two groups were given calcitriol (1,25(OH)₂D3, active vitamin D3, 0.03 μg/kg•d) treatment and peanut oil control treatment, respectively. After 37 days, the samples were collected and the expression of transforming growth factor β1 in rat kidney tissue was detected by RT-PCR, western blot assay and immunohistochemical staining. The expression level of microRNA-130b in kidney was detected by RT-PCR. Morphological structure and degree of fibrosis of rat kidney were analyzed by hematoxylin-eosin staining and Masson staining.

RESULTS AND CONCLUSION: (1) RT-PCR results showed that the expression levels of microRNA-130b in the diabetic nephropathy+ 1,25(OH)₂D3 group and diabetic nephropathy+peanut oil group were significantly lower than those in the normal control group (P < 0.01). The level of microRNA-130b in the diabetic nephropathy+1,25(OH)₂D3 was significantly higher than that in the diabetic nephropathy+peanut oil group (P < 0.01). (2) RT-PCR, western blot assay and immunohistochemical staining and pathological findings showed that the expression level of transforming growth factor β1 mRNA protein and renal tissue structure disorder and degree of fibrosis in the diabetic nephropathy+1,25(OH)₂D3 group and diabetic nephropathy+peanut oil group were significantly higher than those in the normal control group (P < 0.01). The level of transforming growth factor mRNA protein and renal tissue structure disorder and degree of fibrosis in the diabetic nephropathy+1,25(OH)₂D3 group were significantly lower than those in the diabetic nephropathy+peanut oil group (P < 0.01). (3) These results suggest that the expression level of microRNA-130b is decreased and transforming growth factor β1 mRNA and protein levels are increased in renal tissue of diabetic nephropathy rats and the renal tissue structure is disordered and the degree of fibrosis is severe. 1,25(OH)₂D3 can up-regulate the expression of microRMA-130b and also down-regulate the expression of transforming growth factor β1 in the kidney of diabetic nephropathy rats, thus improving the degree of renal tissue structure disorder and fibrosis.

Key words: diabetic nephropathy, SPF Sprague-Dawley rats, streptozotocin, kidney fibrosis, 1,25(OH)₂D3, microRNA-130b, transforming growth factor β1

CLC Number:

R446：R496

R318

Liu Yuetong, Wang Qin, Yang Ye, Zhu Jun, Abulikemu•Tuerdi. Effects of 1,25(OH)₂D3 on microRNA-130b and transforming growth factor beta 1 in renal tissue of rat models of diabetic nephropathy [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(2): 248-253.

Figures/Tables 7

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Effects of 1,25(OH)₂D3 on microRNA-130b and transforming growth factor beta 1 in renal tissue of rat models of diabetic nephropathy

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