Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (27): 4265-4270.doi: 10.3969/j.issn.2095-4344.2015.27.001

    Next Articles

Establishment of animal models of diabetic cardiomyopathy

Liu Wen-qi1, 2, 3, Dai Hong-yan3, Xing Ming-qing4, Guan Jun3, Wang Yan-ping3   

  1. 1 Qingdao University Medical College, Qingdao 266071, Shandong Province, China
    2 Key Laboratory of Cellular Transplantation, Qingdao Municipal Hospital Affiliated to Qingdao University Medical College, Qingdao 266011, Shandong Province, China
    3 Department of Cardiology, Qingdao Municipal Hospital Affiliated to Qingdao University Medical College, Qingdao 266011, Shandong Province, China
    4 Department of Clinical Laboratory, Qingdao Municipal Hospital Affiliated to Qingdao University Medical College, Qingdao 266011, Shandong Province, China
  • Online:2015-06-30 Published:2015-06-30
  • Contact: Corresponding author: Guan Jun, Department of Cardiology, Qingdao Municipal Hospital Affiliated to Qingdao University Medical College, Qingdao 266011, Shandong Province, China Corresponding author: Wang Yan-ping, Department of Cardiology, Qingdao Municipal Hospital Affiliated to Qingdao University Medical College, Qingdao 266011, Shandong Province, China
  • About author:Liu Wen-qi, Studying for master’s degree, Qingdao University Medical College, Qingdao 266071, Shandong Province, China; Key Laboratory of Cellular Transplantation, Qingdao Municipal Hospital Affiliated to Qingdao University Medical College, Qingdao 266011, Shandong Province, China; Department of Cardiology, Qingdao Municipal Hospital Affiliated to Qingdao University Medical College, Qingdao 266011, Shandong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81200175; the Natural Science Foundation of Qingdao City of China, No. 13-1-4-140-jch

PDF

360

Cited

4

Abstract:

BACKGROUND: Diabetic cardiomyopathy, a serious complication of diabetes, is an important factor of increased mortality in patients with diabetes. Therefore, providing an effective experimental animal model is particularly important for studying the pathogenesis and treatment methods of diabetic cardiomyopathy.
OBJECTIVE: To explore the method of establishing Wistar rat models of diabetic cardiomyopathy.
METHODS: Forty rats were randomly divided into the control group (n=10) and diabetic cardiomyopathy group (n=30). The rats in the diabetic cardiomyopathy group were intraperitoneally injected with 60 mg/kg streptozotocin at a time to establish rat models of diabetic cardiomyopathy. The rats in the control group were given the same dosage of citric acid buffer by the same way. The rats in these two groups were all fed with non-fat high-sugar normal diet.
RESULTS AND CONCLUSION: Compared with the control group, after 3 weeks of injection with streptozotocinin in rat models of diabetic cardiomyopathy,  blood glucose level was significantly increased, myocardial cells arranged in disorder, the nuclei were of different sizes, collagen content in the myocardial tissue was significantly increased, and collagen fibers were thick and disordered. In addition, the mRNA and protein levels of transforming growth factor β1 and type I collagen, two indices reflecting myocardial fibrosis, were markedly increased. These results indicate that intraperitoneally injecting large doses of streptozotocin (60 mg/kg) at a time and feeding with non-fat high-sugar normal diet could establish a stable rat model of type 1 diabetic cardiomyopathy. This method is safe and effective with high feasibility.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Diabetic Cardiomyopathy, Streptozotocin, Fibrosis, Myocardium

CLC Number: