Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (10): 1849-1855.doi: 10.3969/j.issn.2095-4344.2013.10.022

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Salvianolic acid B and tanshinoneⅡA induce bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells

Li Xu1, Wang Ya-ling2   

  1. 1 Hebei North University, Zhangjiakou 075000, Hebei Province, China
    2 Department of Cardiology, First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei Province, China
  • Received:2012-05-22 Revised:2012-07-24 Online:2013-03-05 Published:2013-03-05
  • Contact: Wang Ya-ling, Master, Department of Cardiology, First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei Province, China
  • About author:Li Xu★, Studying for master’s degree, Physician, Hebei North University, Zhangjiakou 075000, Hebei Province, China lixubfxy@163.com

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Abstract:

BACKGROUND: To date, most researchers use 5-azacytidine to induce the bone marrow mesenchymal stem cells to differentiate into cardiomyocytes, and there are certain adverse reactions which are difficult to be applied in the clinic. Chinese medicine Danshen is widely used in the clinical treatment of cardiovascular diseases, and its main chemical components are salvianolic acid B and tanshinoneⅡA
OBJECTIVE: To explore the effect of salvianolic acid B and tanshinoneⅡA in inducing bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells.
METHODS: Bone marrow mesenchymal stem cells of Sprague-Dawley rats were isolated and cultured from rat bone marrow, and then the second generation of bone marrow mesenchymal stem cells were induced by salvianolic acid B, tanshinoneⅡA and their combination. The cells without inductor were considered as blank control group. After cultured for 3 days, the induction medium was removed and cultured with normal culture medium for 4 weeks.
RESULTS AND CONCLUSION: Desmin, α-sarcomeric actin, cardiac troponin T and connexin 43 in the blank control group showed weakly positive or negative expression. Compared with the blank control group, the positive expressions of desmin, α-sarcomeric actin, cardiac troponin T and connexin 43 in salvianolic acid B group, tanshinoneⅡA group and salvianolic acid B + tanshinoneⅡA group were significantly increased, and the differences were significant (P < 0.01). The positive expression rate of the markers above was highest in the salvianolic acid B + tanshinoneⅡA group. Fluorescent immunocytochemistry staining showed that the expression of desmin in the cells of induction groups was red and the expression of cardiac troponin T was green, while the part that overlaps was yellow. The results showed that salvianolic acid B and tanshinoneⅡA may induce bone marrow mesenchymal stem cells to acquire cardiogenic phenotype separately, and the effect of combined induction is better than single ingredient.

Key words: stem cells, stem cells and traditional Chinese medicine, salvianolic acid B, tanshinoneⅡA, bone marrow mesenchymal stem cells, cardiomyocytes, differentiation, desmin, α-sarcomeric actin, cardiac troponin T, connexin 43, stem cell photographs-containing paper

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