Glial cell line-derived neurotrophic factor promotes neuron-like cell differentiation of mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.10.023

Abstract

Abstract:

BACKGROUND: The ultimate purpose for inducing the bone marrow mesenchymal stem cells to differentiate into neuron-like cells in vitro is to transplant the induced cells into the body and repair the damaged nervous system. So, it is important to ensure the activity of the transplanted cells.
OBJECTIVE: To investigate the protective effect of glial cell line-derived neurotrophic factor on neuron-like cells differentiated from mucaca multta bone marrow mesenchymal stem cells induced with cryptotanshinone in vitro.
METHODS: The passage 8 mucaca multta bone marrow mesenchymal stem cells were induced with cryptotanshinone to differentiate into neuron-like cells, and the percentage of apoptosis of the induced cells at different time points after induction was detected with flow cytometry (0.5 hour was one group, a total of 12 hours). The period with higher apoptosis percentage was selected to observe the effect of different concentrations of glial cell line-derived neurotrophic factor (0-100 μg/L, totally eleven groups) on the apoptosis of the induced cells.
RESULTS AND CONCLUSION: Cell apoptosis percentage after induction was increased gradually over time and reached a peak about 4 hours, and decreased after 1 hour (P < 0.05). Cell apoptosis percentage was decreased gradually when the concentration of glial cell line-derived neurotrophic factor increased from 0 μg/L to 30 μg/L (P < 0.05), and more remarkable change were not found when glial cell line-derived neurotrophic factor concentration reached 30 μg/L. The results show that glial cell line-derived neurotrophic factor has the protective effect on the neuron-like cells differentiated from mucaca multta bone marrow mesenchymal stem cells induced with cryptotanshinone in vitro.

Key words: stem cells, stem cells and traditional Chinese medicine, glial cell line-derived neurotrophic factor, cryptotanshinone, induction, differentiation, bone marrow mesenchymal stem cells, neuron-like cells, apoptosis, inducing agent, flow cytometry, mucaca multta, stem cell photographs-containing paper

CLC Number:

R394.2

Liu Xiao-gang, Deng Yu-bin, Cai Hui. Glial cell line-derived neurotrophic factor promotes neuron-like cell differentiation of mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(10): 1856-1861.

Figures/Tables 5

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