Serum-containing drugs responsible for supplementing qi and activating blood circulation influences proliferation of olfactory ensheathing cells and secretion of related cytokines

doi:10.3969/j.issn.2095-4344.2013.06.024

Abstract

Abstract:

BACKGROUND: Supplementing qi and removing blood stasis is the main therapeutic principle of traditional Chinese medicine for the treatment of spinal cord injury, but the efficacy is not plausible. But, whether the application of serum-containing drugs responsible for supplementing qi and activating blood circulation under the guidance of modern technology can treat spinal cord injury and improve the efficacy?
OBJECTIVE: To study the effect of serum-containing drugs responsible for supplementing qi and activating blood circulation on the proliferation of olfactory ensheathing cells and the secretion of nerve growth factor and brain-derived neurotrophic factor.
METHODS: The olfactory ensheathing cells were in vitro cultured with tissue block culture method. The effect of Buyanghuanwu decoction and Weizheng decoction on the proliferation of olfactory ensheathing cells was detected with MTT assay. The expression levels of nerve growth factor and brain-derived neurotrophic factor in the culture supernatant were detected by western blot assay.
RESULTS AND CONCLUSION: MTT results showed that both Buyanghuanwu decoction and Weizheng decoction could promote the proliferation of olfactory ensheathing cells, and there was no significant difference in proliferation-promoting effect. Western blot assay results showed that both Buyanghuanwu decoction and Weizheng decoction could promote the expression of nerve growth factor and brain-derived neurotrophic factor in olfactory ensheathing cells, and there was no significant difference in secretion level between two groups.

Key words: stem cells, stem cells and traditional Chinese medicine, culture and differentiation, Buyanghuanwu decoction, Weizheng decoction, supplementing qi and activating blood circulation, drug-containing serum, olfactory ensheathing cells, proliferation, nerve growth factor, brain-derived neurotrophic factor, proliferation promotion, tissue repair, photographs-containing paper of stem cells

CLC Number:

R394.2

Rao Yao-jian, Zhu Wen-xiao, Ma Wen-hu, Wang Yong-jun. Serum-containing drugs responsible for supplementing qi and activating blood circulation influences proliferation of olfactory ensheathing cells and secretion of related cytokines[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(6): 1094-1100.

Figures/Tables 7

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