Effects of cerebrolysin on cultured neuroglia antigen 2-positive neural progenitor cells

doi:10.3969/j.issn.2095-4344.2013.06.022

Abstract

Abstract:

BACKGROUND: Cerebrolysin can relieve the symptoms of many nervous system diseases, but the precise mechanism remains poorly understood. Cerebrolysin has been shown to promote neurogensis by in vivo and in vitro experiments.
OBJECTIVE: To investigate the effects of cerebrolysin on the proliferation and differentiation of cultured neuroglia antigen 2-positive neural progenitor cells.
METHODS: Neuroglia antigen 2 cells from the hippocampus of the adult rats were primary cultured and sub-cultured. Cell characteristics were identified by immunofluorescence staining. Cell viability was determined by lactate dehydrogenase assay. Cell apoptosis was detected by TUNEL method. The newly generated cells were identified by BrdU incorporation.
RESULTS AND CONCLUSION: Cerebrolysin significantly decreased the number of TUNEL-positive cells and significantly increased the number of neuroglia antigen 2-positive cells and the expression level of microtubule-associated protein 2a+2b, synapsin I, γ-aminobutyric acid and vesicularγ-aminobutyric acid transporter in neuroglia antigen 2-positive cells. These findings suggest that cerebrolysin can inhibit neuroglia antigen 2-positive cell apoptosis, promote neuroglia antigen 2-positive cell proliferation and differentiation into neurons (especially GABAergic inhibitory interneurons).

Key words: stem cells, stem cell culture and differentiation, cerebrolysin, neuroglia antigen 2, neural progenitor cell, proliferation, differentiation, lactate dehydrogenase, lactate dehydrogenase, microtubule-associated protein 2a+2b, γ-aminobutyric acid, vesicularγ-aminobutyric acid transporter, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Yu Xiu-jun, Li Yi, Wen Di. Effects of cerebrolysin on cultured neuroglia antigen 2-positive neural progenitor cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(6): 1081-1088.

Figures/Tables 8

References

[1] Belachew S, Chittajallu R, Aguirre AA,et al. Postnatal NG2 proteoglycan-expressing progenitor cells are intrinsically multipotent and generate functional neurons.J Cell Biol. 2003;161(1):169-186.

[2] Nunes MC, Roy NS, Keyoung HM,et al.Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain.Nat Med. 2003;9(4):439-447.

[3] Lin SC, Huck JH, Roberts JD,et al. Climbing fiber innervation of NG2-expressing glia in the mammalian cerebellum.Neuron. 2005;46(5):773-785.

[4] Levine JM, Reynolds R, Fawcett JW.The oligodendrocyte precursor cell in health and disease.Trends Neurosci. 2001; 24(1):39-47.

[5] Wennström M, Hellsten J, Ekdahl CT,et al. Electroconvulsive seizures induce proliferation of NG2-expressing glial cells in adult rat hippocampus.Biol Psychiatry. 2003;54(10): 1015-1024.

[6] Wennström M, Hellsten J, Tingström A. Electroconvulsive seizures induce proliferation of NG2-expressing glial cells in adult rat amygdala. Biol Psychiatry. 2004;55(5):464-471.

[7] Gschanes A, Valousková V, Windisch M. Ameliorative influence of a nootropic drug on motor activity of rats after bilateral carotid artery occlusion. J Neural Transm. 1997; 104(11-12):1319-1327.

[8] Tatebayashi Y, Lee MH, Li L,et al. The dentate gyrus neurogenesis: a therapeutic target for Alzheimer's disease. Acta Neuropathol. 2003;105(3):225-232.

[9] Yu XJ,Li ZZ,Guo L,et al. Nao yu Shenjing Jibing Zazhi. 2009; 17(2):93-96. 于秀军,李震中,郭力,等.从成年大鼠中枢神经系统不同区域分离NG2蛋白聚糖阳性神经祖细胞[J].脑与神经疾病杂志,2009, 17(2): 93-96.

[10] Buffo A, Vosko MR, Ertürk D,et al. Expression pattern of the transcription factor Olig2 in response to brain injuries: implications for neuronal repair.Proc Natl Acad Sci U S A. 2005;102(50):18183-18188.

[11] Greenwood K, Butt AM.Evidence that perinatal and adult NG2-glia are not conventional oligodendrocyte progenitors and do not depend on axons for their survival.Mol Cell Neurosci. 2003;23(4):544-558.

[12] Mohapel P, Frielingsdorf H, Häggblad J,et al. Platelet-derived growth factor (PDGF-BB) and brain-derived neurotrophic factor (BDNF) induce striatal neurogenesis in adult rats with 6-hydroxydopamine lesions. Neuroscience. 2005;132(3): 767-776.

[13] Jacobs BL, van Praag H, Gage FH.Adult brain neurogenesis and psychiatry: a novel theory of depression.Mol Psychiatry. 2000;5(3):262-269.

[14] Bogen IL, Boulland JL, Mariussen E,et al. Absence of synapsin I and II is accompanied by decreases in vesicular transport of specific neurotransmitters.J Neurochem. 2006; 96(5):1458-1466.

[15] Lovell MA, Geiger H, Van Zant GE,et al. Isolation of neural precursor cells from Alzheimer's disease and aged control postmortem brain.Neurobiol Aging. 2006;27(7):909-917.

[16] Sugaya K, Alvarez A, Marutle A,et al. Stem cell strategies for Alzheimer's disease therapy.Panminerva Med. 2006;48(2): 87-96.

[17] Torrey EF, Barci BM, Webster MJ,et al. Neurochemical markers for schizophrenia, bipolar disorder, and major depression in postmortem brains.Biol Psychiatry. 2005; 57(3):252-260.

[18] Stanley DP, Shetty AK.Aging in the rat hippocampus is associated with widespread reductions in the number of glutamate decarboxylase-67 positive interneurons but not interneuron degeneration.J Neurochem. 2004;89(1):204-216.