Oriented differentiation of human umbilical cord mesenchymal stem cells into islet-like cells in vitro

doi:10.3969/j.issn.2095-4344.2013.06.011

Abstract

Abstract:

BACKGROUND: Human umbilical cord mesenchymal stem cells have some advantages such as easy acquirement and low immunity. But there is no mature program for differentiating into islet-like cells in vitro at present.
OBJECTIVE: To investigate the possibility of human umbilical cord mesenchymal stem cells differentiating into islet-like cells in appropriate conditions.
METHODS: The human umbilical cord mesenchymal stem cells were isolated and cultured under sterile conditions, following passage for three generations. The human umbilical cord mesenchymal stem cells were induced to differentiate into islet-like cells by two steps. Step 1: the cells were cultured in the dulbecco’s modified eagle’s medium/F12 culture medium containing 100 μg/L β-nerve growth factors, 4 nmol/L activinA, 10 mmol/L nicotinamide, 25 μg/L epidermal growth factor and fetal bovine serum with the volume fraction of 10% for 7 days. Step 2: the induction medium was changed into the dulbecco’s modified eagle’s medium /F12 culture medium containing 10 mmol/L nicotinamide, 10 μg/L alkaline fibroblast growth factors and 1% insulin-transferrin-selenium, and induced for 14 days. The cells in the control group were cultured with the dulbecco’s modified eagle’s medium/F12 culture medium.
RESULTS AND CONCLUSION: Cells began to aggregate after induced for 2 weeks. After 3 weeks of induction, the glucose stimulation test was positive with the expression of pancreatic and duodenal homeobox 1 and insulin. No insulin secretion was found in the control group and the expression of islet cells related gene was negative in the control group. The human umbilical cord mesenchymal stem cells were successfully induced to differentiate into the islet-like cells.

Key words: stem cells, stem cell culture and differentiation, islet-like cells, human umbilical cord mesenchymal stem cells, differentiation, islet, pancreatic and duodenal homeobox 1, other grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Yu Ya-bin, Chen Wei, Bian Jian-min. Oriented differentiation of human umbilical cord mesenchymal stem cells into islet-like cells in vitro[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(6): 1012-1017.

Figures/Tables 5

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