Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (2): 309-314.doi: 10.3969/j.issn.2095-4344.2013.02.022

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Procyanidins effects on apoptosis-related proteins in the gastrocnemius after heavy load training in rats

Min Zhu1, Liu Wen-jie2, Geng Xiao2, Su Quan-sheng3   

  1. 1  College of Physical Education, Nanjing Normal University, Nanjing 210000, Jiangsu Province, China
    2  Graduate School, Chengdu Sport University, Chengdu 610041, Sichuan Province, China
    3  Department of Sports Biomechanics, Chengdu Sport University, Chengdu 610041, Sichuan Province, China
  • Received:2012-03-15 Revised:2012-05-30 Online:2013-01-08 Published:2013-01-08
  • About author:Min Zhu★, Master, Lecturer, College of Physical Education, Nanjing Normal University, Nanjing 210000, Jiangsu Province, China 413347660@qq.com

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Abstract:

BACKGROUND: It will bring a variety of negative effects to the body after heavy load training, which is related to delayed onset muscle soreness and exercise fatigue.
OBJECTIVE: To observe the effect of procyanidins on metabolism of free radicals, apoptosis-related protein FAS, and tumor necrosis factor in the gastrocnemius after heavy load training in rats.
METHODS: Totally 48 adult female Sprague-Dawley rats were randomly divided into placebo group and medication group, and each group was divided into three subgroups, including static control group, instantly post-exercise group and 24-hour post-exercise group. Rats of the medication group were fed with 10 g/L procyanidins by intragastric administration, and those in the placebo group were given equal volume of distilled water as placebo. After 2 weeks, in all groups expect two static control groups, a heavy load running with a slope of -10° at 20 m/min was performed. Rats were decapitated before exercise, instantly and 24 hours after exercise, respectively, and then gastrocnemius from the left hind limb was taken off for detection.
RESULTS AND CONCLUSION: (1) The gastrocnemius superoxide dismutase activity had a decrease tendency in the two instantly post-exercise groups, and the decline in superoxide dismutase activity was slighter in the medication group than the placebo group. The content of malondialdehyde increased significantly in the two instantly post-exercise groups, but the increase was smaller in the medication group than the placebo group. The ratio of superoxide dismutase and malondialdehyde decreased significantly after exercise, but compared with the placebo group, the decrease of the medication group was lower at every phase after exercise. (2) The expression of FAS protein in both groups increased significantly after exercise, and the increase of the placebo group was higher than the medication group. (3) The expression of tumor necrosis factor α was higher in the placebo group than the medication group at different phase after exercise. These findings indicate that to a certain extent, procyanidins could relieve the injury of gastrocnemius and restrain cell apoptosis after a heavy load exercise in rats.

Key words: tissue construction, cytological experiments of tissue construction, heavy load exercise, procyanidins, gastrocnemius, apoptosis, free radical, tissue construction photographs-containing paper

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