Existence and features of Telocyte cells in the mouse heart

doi:10.3969/j.issn.2095-4344.1586

Abstract

Abstract:

BACKGROUND: Studies have found that Telocyte interstitial cells may play a role in heart diseases, such as acute myocardial infarction, atrial fibrillation, valvular heart disease and myocardial amyloidosis, but the mechanism is unclear. The basic research of cardiac Telocyte interstitial cells is of important clinical value.
OBJECTIVE: To explore the existence and characteristics of Telocyte interstitial cells in the mouse heart.
METHODS: Fifty-nine Kunming mice aged from 10 days to 10 weeks (provided by Fuzhou Wu’s Experimental Animal Center in China) were chosen. The gastrointestinal tract Cajal interstitial cells and bladder Telocyte interstitial cells were used as controls. ACK2 (c-kit monoclonal antibody) was used as an indicator for the immunohistochemical staining of myocardial tissue in 13 mice. Cardiac Telocyte cells from another 41 mice were isolated, cultured in vitro and observed for cell morphology and protrusion under immunofluorescence laser confocal microscope. Ultrastructure of Cajal and Telocyte interstitial cells from the remaining five mice was observed by transmission electron microscopy.
RESULTS AND CONCLUSION: (1) Positive expression of c-kit was found in the gastrointestinal and bladder tissues by immunohistochemical staining, but not in the cardiac tissues. However, in vitro cultured cells were positive for c-kit. (2) Under the immunofluorescence laser confocal microscope and transmission electron microscope, the cardiac Telocyte cells with spindle or irregular bodies were discovered in myocardial fibers or around the capillaries, and dense strips were found between cell protrusions from cardiac Telocyte cells and the capillaries. There was no difference in the shape of cell body and the number of cell protrusion compared with the control group, but a significant difference in the length of cell protrusions was found (P < 0.05). In conclusion, the presence of Telocyte cells with positive expression of c-kit in the mouse heart is confirmed by primary culture, immunofluorescence staining and transmission electron microscopy. The body size and number of cell protrusions are similar to those of gastrointestinal tract Cajal interstitial cells or bladder Telocyte interstitial cells. Ventricular Telocyte interstitial cell protrusion is the longest, atrial Telocyte interstitial cell protrusion is the shortest, and ventricular Telocyte interstitial cell protrusion is tightly connected with the capillaries.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stromal Cells, Mice, Microscopy, Fluorescence, Tissue Engineering

CLC Number:

Deng Lizhu, Huang Qianwen, Noman Qureshi, Li Juxiang, Duan Shaoyin. Existence and features of Telocyte cells in the mouse heart[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(5): 784-789.

Figures/Tables 3

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