Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (6): 951-956.doi: 10.3969/j.issn.2095-4344.1540

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Preparation of acellular vascular scaffold using Triton-x100 and salvianolic acid B and its biomechanical performance

Zhao Liang1, 2, Li Xiafei3, Zhou Kun2, Yan Huanhuan2, Zhang Qiqing2   

  1. 1College of Engineering, Peking University, Beijing 100871, China; 2School of Life Sciences and Technology, Life Science and Health Research Institute, Xinxiang Medical University, Xinxiang 453003, Henan Province, China; 3School of Biomedical Engineering, Xinxiang Medical University, Xinxiang 453003, Henan Province, China
  • Received:2018-10-25 Online:2019-02-28 Published:2019-02-28
  • Contact: Zhao Liang, College of Engineering, Peking University, Beijing 100871, China; School of life Sciences and Technology, Life Science and Health Research Institute, Xinxiang Medical University, Xinxiang 453003, Henan Province, China
  • About author:Zhao Liang, PhD, College of Engineering, Peking University, Beijing 100871, China; School of Life Sciences and Technology, Life Science and Health Research Institute, Xinxiang Medical University, Xinxiang 453003, Henan Province, China
  • Supported by:

    The Key Scientific Research Project of Henan Higher Education Institutions, No. 17A180034 (to ZL); the Project of the Second College Student Life Sciences League, No. SKYLS017 (to ZL)

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Abstract:

BACKGROUND: The acellular vascular scaffold has excellent properties, but its biomechanical properties have been reduced to varying degrees during preparation.

OBJECTIVE: To explore the application prospect of salvianolic acid B in the tissue-engineered preparation of acellular vascular scaffold.
METHODS: The rat thoracic aorta was decellularized using Triton-x100 alone (Tx group) or combined with salvianolic acid B (Tx-sal group) to prepare acellular vascular scaffolds. Morphology of the two groups of acellular vascular scaffolds was observed. Measurements of tissue thickness, porosity and biomechanical properties were conducted, and changes in the morphology of acellular vascular scaffolds were compared between two groups.
RESULTS AND CONCLUSION: The use of salvianolic acid B treatment had no effect on the wall thickness and porosity of acellular vascular scaffolds, and there was no significant difference between the Tx group and Tx-sal group (P > 0.05). There was also no significant change in the scaffold morphology. Compared with the Tx group, the acellular vascular scaffold in the Tx-sal group was better to retain collagen fibers and elastic fibers of the vessel wall. The biomechanical indicators in the Tx-sal gorup such as tensile strength, maximum deformation, elongation at break, burst pressure, and suture strength were all superior to those in the Tx group (P < 0.05). Therefore, Triton-x100 combined with salvianolic acid B treatment can produce acellular vascular materials with good decellularization and excellent biomechanical properties. 

Key words: Stents, Biomechanics, Tissue Engineering

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