In vitro enzymatic degradation of poly(trimethylene carbonate): influencing factors and their effects

doi:10.3969/j.issn.2095-4344.1556

Abstract

Abstract:

BACKGROUND: Poly(trimethylene carbonate) has great potential in clinical applications due to the excellent biocompatibility and biodegradability. Little is reported on the factors influencing the in vitro enzymatic degradation of poly(trimethylene carbonate) and the underlying mechanism.

OBJECTIVE: To investigate the in vitro enzymatic degradation of poly(trimethylene carbonate), and to explore the influencing factors and their effects on the poly(trimethylene carbonate) degradation.

METHODS: poly(trimethylene carbonate) homopolymers and copolymers were prepared by ring-opening polymerization. The molecular mass of the two homopolymers was 135 and 256 kDa, respectively. The molecular mass of the copolymers was 238 kDa. The homopolymer of 256 kDa was made into two shapes (rods and films), and the other two samples were shaped into rods. The in vitro enzymatic degradation of poly(trimethylene carbonate) was conducted in lipase solutions, the homopolymer samples were taken out at 1, 2, 4, 8, 10, and 12 weeks, and the copolymer samples were taken at 3, 6, 9, 12, and 15 days. The mass loss and degradation rate constant were measured.

RESULTS AND CONCLUSION: The molecular mass and shape as well as molar ratio played important roles on the in vitro enzymatic degradation behavior of poly(trimethylene carbonate). As the molecular mass increased from 135 to 256 kDa, the degradation rate constant of poly(trimethylene carbonate) homopolymer increased from 1.46% to 3.81%, indicating that the higher the molecular mass, the higher degradation rate of poly(trimethylene carbonate). The poly(trimethylene carbonate) film presented with higher degradation rate than the cylinder one with the same molecular weight, and the degradation rate constant increased from 3.81% to 9.16% as the shape of poly(trimethylene carbonate) with a molecular weight of 256 kDa changed from rods to films. The introduction of polycaprolactone segment accelerated the degradation rate of poly(trimethylene carbonate). The degradation rate constant increased from 3.81% to14.49% as the 50 mol% caprolactone content was introduced into the structure of poly(trimethylene carbonate) (256 kDa). In summary, the order of factors influencing the degradation rate of poly(trimethylene carbonate) is as follows: copolymer composition > shape > molecular mass.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Materials Testing, Lipase, Tissue Engineering

CLC Number:

Li Wuyuntana, Zhao Lingyan, Zhang Wei, Xia Yuan, Yang Liqun. In vitro enzymatic degradation of poly(trimethylene carbonate): influencing factors and their effects[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(6): 945-950.

Figures/Tables 7

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