Effect of gelatin sponge/beta-tricalcium phosphate composite with different doses of basic fibroblast growth factor on bone regeneration

doi:10.3969/j.issn.2095-4344.1506

Abstract

Abstract:

BACKGROUND: The current method of improving tissue regeneration is to combine growth factors with scaffold materials. Therefore, more and more attention has been paid to the gene-enhanced tissue engineering method.

OBJECTIVE: To evaluate the effect of gelatin sponge/β-tricalcium phosphate composite mixed with different doses of basic fibroblast growth factor on bone regeneration.

METHODS: Forty male Sprague-Dawley rats were selected to prepare skull defect models. Rat models were randomly divided into four groups, and implanted with gelatin sponge/β-tricalcium phosphate composite, gelatin sponge carrying 10, 100 and 200 ng of basic fibroblast growth factor/β-tricalcium phosphate composite. After 8 weeks of implantation, bone formation was observed by gross and histological observations.

RESULTS AND CONCLUSION: In each group, the gelatin sponge/β-tricalcium phosphate composite was encapsulated by a large number of fibrous connective tissues, a large number of neovascularization and lymphocytes distributed in the fibrous connective tissue, and macrophages were visible around β-tricalcium phosphate granules. β-tricalcium phosphate granules were not obviously absorbed, and there was new bone formation between β-tricalcium phosphate and the skull. Many active bone cells existed among the new bone trabeculae, with disordered woven bone structure, the mature lamellar bone underneath the woven bone, and the undamaged original skull. The most active new bone formation was found in the basic fibroblast growth factor 100 ng+gelatin sponge/β-tricalcium phosphate composite group, and the new bone volume was the least in the solely used gelatin sponge/β-tricalcium phosphate composite. In conclusion, the combination of basic fibroblast growth factor with gelatin sponge/β-tricalcium phosphate composite can promote bone regeneration in the bone defect, and the best effect on bone regeneration is observed when 100 ng basic fibroblast growth factor is used.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Fibroblast Growth Factor 2, Calcium Phosphates, Bone Regeneration, Tissue Engineering

CLC Number:

R459.9

R318

Cui Tingting, Qiu Zewen, Gui Lin, Zhong Weijian, Ma Guowu. Effect of gelatin sponge/beta-tricalcium phosphate composite with different doses of basic fibroblast growth factor on bone regeneration[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(2): 190-195.

Figures/Tables 4

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