Cytocompatibility of injectable glycol chitosan/dibenzaldehyde-terminated poly-ethyleneglycol hydrogel

doi:10.3969/j.issn.2095-4344.1507

Abstract

Abstract:

BACKGROUND: Our research group independently developed an injectable glycol chitosan/dibenzaldehyde-terminated poly-ethyleneglycol (GCS/DF-PEG) hydrogel, which has good injectability and self-healing properties.

OBJECTIVE: To test the physical properties and cytocompatibility of the GCS/DF-PEG hydrogel.

METHODS: The injectable GCS/DF-PEG hydrogel was prepared by mixing GCS solution at a mass fraction of 1.5% with an equal volume of DF-PEG solution at a mass fraction of 2%, 4%, and 8%, respectively. Their moduli of elasticity were measured. Three groups of injectable hydrogels were immersed in PBS for 4 weeks to detect the in vitro degradation of the hydrogels. Passage 2 adipose-derived mesenchymal stem cells from Sprague-Dawley neonatal rats were cultured in injectable GCS/DF-PEG hydrogel leaching solution as experimental group or cultured routinely as control group. MTT assay was used to detect the cell proliferation. Passage 2 adipose-derived mesenchymal stem cells from Sprague-Dawley neonatal rats were mixed with 3% GCS solution, and then mixed with 4% DF-PEG solution. On the 1^st and 5^thdays of culture, the cell survival and death in the hydrogel were tested by live/dead staining. Passage 2 adipose-derived mesenchymal stem cells from Sprague-Dawley neonatal rats were mixed with 3% GCS solution, and then mixed with 2%, 4% and 8% DF-PEG solution, respectively. MTT method was used for testing the cell proliferation.

RESULTS AND CONCLUSION: (1) The moduli of elasticity of GCS/DF-PEG hydrogel with 2%, 4%, 8% DF-PEG were 13.48, 22.21 and 33.19 kPa, respectively. (2) In vitro degradation experiments showed that GCS/DF-PEG hydrogels gradually degraded in PBS over time. And the degradation rate of the 2% DF-PEG hydrogel was significantly faster than the other two groups. (3) Within 7 days of culture, there was no difference in the cell proliferation between the experimental and control groups. (4) Live/dead staining results showed that adipose-derived mesenchymal stem cells were spherical in the hydrogel, the survival rate was over 90%, and the number of cells increased significantly with time. (5) Over time, the number of adipose-derived mesenchymal stem cells in the GCS/DF-PEG hydrogel with different mass fractions gradually increased. And the cell proliferation in the hydrogel containing 2% DF-PEG was faster than 4% and 8% groups (P < 0.05). In conclusion, the injectable GCS/DF-PEG hydrogel has good cytocompatibility, mechanical strength and degradation, and it is expected to be a good carrier for stem cell transplantation in cartilage tissue engineering.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Hydrogel, Materials Testing, Cell Proliferation, Tissue Engineering

CLC Number:

R459.9
R318

Jing Xiaoguang, Liu Shuyun, Guo Weimin, Li Xu, Lü Ao, Liu Shichen, Meng Haoye, Chen Mingxue, Zhang Xueliang, Zhang Zengzeng, Liu Xuejian, Gao Chao, Wang Zehao, Zhang Bin, Shen Shi, Tao Lei, Yang Jianhua, Guo Quanyi. Cytocompatibility of injectable glycol chitosan/dibenzaldehyde-terminated poly-ethyleneglycol hydrogel[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(2): 196-203.

Figures/Tables 9

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