Establishment of abdominal cardiac transplant model in mice and technical modifications

doi:10.3969/j.issn.2095-4344.1390

Abstract

Abstract:

BACKGROUND: The mouse abdominal heart transplantation model is an important platform for studying immune tolerance. Although various modified methods have been reported, microsurgical vascular anastomosis is required. The authors have reported a series of modified methods for end-to-side anastomosis of mouse abdominal heart transplantation, and the modified technique is applicable to other microvascular end-to-side anastomosis.
OBJECTIVE: To establish a stable, reliable and manageable abdominal heterotopic cardiac transplant model in mice.
METHODS: One hundred and forty-four C57/BL6J (H-2b), DBA/2 (H-2d), and Balb/C (H-2d) mice were as donors, n=48 per specie, and 144 Balb/C (H-2d) mice were as recipient mice. All mice were provided by Beijing Weitong Lihua Company, and the study was approved by the Experimental Animal Ethics Committee of Gansu Provincial Hospital (approval No. 2017-011). There were two groups, modified and traditional groups, and 72 pairs of mice underwent cardiac transplant. Abdominal heterotopic cardiac transplantation in mice was performed using the following techniques: donor heart was harvested by a modified approach; recipient blood vessels were blocked by a self-made arched vascular clamp; incision was made on the anterior wall of blood vessels using an 11-0 suture needle, and pulmonary artero-inferior vena cava anastomosis was established using 170-degree dual-fixation method. The venous anastomosis time and success rate of transplantation were compared.
RESULTS AND CONCLUSION: (1) The success rate of transplantation in the modified group was 90.3%, the donor procedure time was (7.0±0.5) minutes, the recipient procedure time was (53.5±5.9) minutes, blood vessel blockage time was (30.4±4.5) minutes, and venous anastomosis time was (9.4±1.5) minutes. The success rate of transplantation in the traditional group was 86.1%; the donor procedure time was (12.3±2.1) minutes; the recipient procedure time was (80.0±7.1) minutes; blood vessel blockage time was (45.6±6.0) minutes; and venous anastomosis time was (14.0±2.3) minutes. The modified group was superior to the traditional group in the transplantation time and success rate (P < 0.01 or P < 0.05). (2) These results indicate that the modified approaches speed up the abdominal cardiac transplantation in mice and decrease the complexity of the surgery, and establish a stable and reliable abdominal heterotopic cardiac transplant model in mice.

Key words: heterotopic heart transplantation, abdominal, model, mice, technical modifications, 170-degree dual-fixation method, arterial anastomosis

CLC Number:

Li Junliang1, 2, Zhang Dong3, Guo Tiankang1, 2, Tian Hongwei1, Wang Tao1. Establishment of abdominal cardiac transplant model in mice and technical modifications [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(27): 4387-4391.

Figures/Tables 2

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