Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (27): 4326-4331.doi: 10.3969/j.issn.2095-4344.1380

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Asparaginase can promote the avascular necrosis of femoral head induced by dexamethasone in mouse models

Li Minde1, Yang Fan2, Chen Haojie1, Li Shaopeng1, Ma Shengli1, Xiao Peng1, Liu Baoyi1   

  1.  (1First Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China; 2Department of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian 116024, Liaoning Province, China)
  • Received:2019-03-26 Online:2019-09-28 Published:2019-09-28
  • Contact: Liu Baoyi, MD, Associate chief physician, First Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
  • About author:Li Minde, Master candidate, Physician, First Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
  • Supported by:

    the National Postdoctoral Scientific Research Project of China, No. 171480 (to LBY); the General Project of National Postdoctoral Scientific Foundation of China, No. 2017M621116 (to LBY); the Doctoral Research Startup Foundation of Liaoning Province, No. 201601299 (to LBY); the General Project of Scientific Research of Education Department of Liaoning Province, No. L2015021 (to LBY); the High-Level Talent Innovation Support Program- “Science Star” Project of Dalian, No. 2017RQ154 (to LBY)

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Abstract:

BACKGROUND: Asparaginase can inhibit angiogenesis and affect blood supply, and make damage to the vascular endothelial cells, thereby affecting the local blood supply of femoral head.
OBJECTIVE: To investigate the effect of asparaginase on the avascular necrosis of femoral head induced by dexamethasone.
METHODS: Balb/c male mice were provided by Laboratory Animal Center of Dalian Medical University, and the study was approved by the Experimental Ethics Committee of Affiliated Zhongshan Hospital of Dalian University. Sixty male Balb/c mice were randomly divided into four groups: asparaginase + dexamethasone group (n=15, 2 mg/L dexamethasone in the water, and intraperitoneal injection of 1 200 U/kg asparaginase), asparaginase group (n=15, intraperitoneal injection of 1 200 U/kg asparaginase), dexamethasone group (n=15, 2 mg/L dexamethasone in the water), and control group (n=15, no intervention). All mice were killed after 8 weeks. The body mass, coagulation factors, hematoxylin-eosin staining and immunohistochemical staining of the femoral head were observed.
RESULTS AND CONCLUSION: (1) The body mass gain in the asparaginase + dexamethasone group was significantly lower than that in the other three groups. (2) The levels of coagulation factor III and V in the asparaginase + dexamethasone group were significantly higher than those in the other three groups (P < 0.05 or P < 0.01). (3) Compared with the other three groups, the empty bone lacunae rate was increased (P < 0.05), and trabecular fracture rate was decreased (P < 0.05), and the degree of osteonecrosis was more obvious in the asparaginase + dexamethasone group. (4) The absorbance value of osteoprotegerin in the asparaginase + dexamethasone group was significantly lower than that in the other three groups (P < 0.05 or P < 0.01). (5) These results indicate that asparaginase can promote avascular necrosis of femoral head in mice.

Key words: asparaginase, intravascular coagulation, vascular injury, avascular necrosis of the femoral head, mice

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