Co-transplantation of adipose mesenchymal stem cells and endothelial progenitor cells in ulcerative colitis mice

doi:10.3969/j.issn.2095-4344.2110

Abstract

Abstract:

BACKGROUND: Mesenchymal stem cell transplantation has been proved to be effective for ulcerative colitis, while the effect of endothelial progenitor cells in ulcerative colitis treatment is still unknown. They are both promising cells for tissue engineering, which can be used for cell transplantation.

OBJECTIVE: To explore whether co-transplantation of endothelial progenitor cells and adipose mesenchymal stem cells can relieve ulcerative colitis in a mouse model.

METHODS: C57BL/6J mice were randomly divided into six groups (n=24 per group): co-transplantation group, mesenchymal stem cell transplantation group, endothelial progenitor cell transplantation group, glucocorticoid treatment group, transplantation control group and normal control group. Murine ulcerative colitis model was established in all groups except for the normal control group. At 7 and 10 days after modeling, transplantation groups were respectively injected via tail vein with adipose mesenchymal stem cells and/or endothelial progenitor cells, glucocorticoid or PBS. Mice were sacrificed at 12 days after modeling. Colon length, disease activity index, histological score and the serum level of tumor necrosis factor-α were compared between groups.

RESULTS AND CONCLUSION: Treatment with glucocorticoid was significantly effective for ulcerative colitis relative to the transplantation control group (P < 0.05). Adipose mesenchymal stem cells were proved to have better effects than glucocorticoids in the murine ulcerative colitis model (P < 0.05). No significant differences were found between endothelial progenitor cell transplantation group and transplantation control group (P > 0.05). Co-transplantation of adipose mesenchymal stem cells and endothelial progenitor cells was better than the other treatments, which significantly improved the shortening of the colon, disease activity index, histological score, and serum level of tumor necrosis factor-α.

Key words:

adipose mesenchymal stem cells, endothelial progenitor cells, fluorescent label, cell transplantation, C57BL/6J mice, ulcerative colitis

CLC Number:

Hou Xiaolin, Liang Jun, Yang Cheng, Cui Meihua.

Co-transplantation of adipose mesenchymal stem cells and endothelial progenitor cells in ulcerative colitis mice [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(25): 3981-3987.

Figures/Tables 6

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