Effect of collagenase type II on the biomechanical properties of rabbit cornea

doi:10.3969/j.issn.2095-4344.1282

Abstract

Abstract:

BACKGROUND: Many pathological states of the cornea are associated with abnormal expression of collagenase type II, which can cause corneal matrix degradation and may result in the changes of corneal mechanical properties. The mechanical properties of the cornea are closely related to the corneal shape and refractive state.

OBJECTIVE: To observe the changes in the mechanical properties of the rabbit cornea after treatment with collagenase type II.

METHODS: After anesthesia and epithelial debridement, a collagenase type II solution (18 g/L, 200 μL) was used to treat the left eyes of the experimental rabbits for 30 minutes. Then all rabbits were normally raised for 3 months. The experiments were approved by the Ethics Committee of Animal Experiment and Laboratory Animal Welfare of Capital Medical University (approval No. AEEI-2014-066).

RESULTS AND CONCLUSION: Compared with the control eyes, the elastic modulus of the experimental eyes in the low and high strain regions decreased by 51.5% and 29.9%, respectively. Overall, the degree of stress relaxation of the experimental eyes was smaller than that of the control eyes. These results suggest that collagenase type II eads to changes in corneal mechanical properties, decreases in elastic modulus, decreases in the degree of overall stress relaxation, and decreases in the overall carrying capacity of the cornea.

Key words: collagenase type Ⅱ, cornea, mechanical properties, uniaxial stretching, elastic modulus, stress relaxation, stress, strain

CLC Number:

R443
R318

Chen Xinyan, Qin Xiao, Zhang Haixia, Li Lin. Effect of collagenase type II on the biomechanical properties of rabbit cornea[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(22): 3556-3561.

Figures/Tables 6

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