Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (2): 286-291.doi: 10.3969/j.issn.2095-4344.2962

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Effects of baicalin on oxidative stress in BEAS-2B cells stimulated by lipopolysaccharide combined with adenosine triphosphate

Cong Renyuan, Yuan Jing, Xia Jinchan, Sun Ying    

  1. Basic Medical College of Henan University of Chinese Medicine, Zhengzhou  450046, Henan Province, China
  • Received:2020-03-17 Revised:2020-03-21 Accepted:2020-04-21 Online:2021-01-18 Published:2020-11-14
  • Contact: Xia Jinchan, MD, Associate professor, Basic Medical College of Henan University of Chinese Medicine, Zhengzhou 450046, Henan Province, China
  • About author:Cong Renyuan, Master candidate, Basic Medical College of Henan University of Chinese Medicine, Zhengzhou 450046, Henan Province, China
  • Supported by:
    the National Natural Science Foundation of China (Youth Project), No. 81803863; Special Research and Development Project of Henan Provincial Department of Science and Technology Department, No. 192102310163; Henan Provincial Young Teacher Training Program for High Educations, No. 2017GGJS080; 2019 Graduate Research and Innovation Project of Henan University of Chinese Medicine, No. 2019KYCX018

Abstract: BACKGROUND: Oxidative stress and inflammatory reaction play important roles in the occurrence and development of acute lung injury. Studies have shown that baicalin has biological activities such as antioxidant and anti-inflammatory. 
OBJECTIVE: To investigate the effects of baicalin on oxidative stress and TXNIP/NLRP3 pathway in BEAS-2B cells induced by lipopolysaccharide combined with adenosine triphosphate. 
METHODS: The inflammatory model of BEAS-2B cells was established by lipopolysaccharide combined with adenosine triphosphate stimulation. The cells were randomly divided into blank control group, model group and baicalin group (2.5, 5, and 10 mg/L). MTT method was used to detect the cell vitality. ELISA was used to detect the levels of interleukin-1β, interleukin-6, interleukin-18 and tumor necrosis factor-ɑ. The changes of intracellular reactive oxygen species, superoxide dismutase and malondialdehyde were measured by fluorescent probe DCFH-DA and microplate reader, respectively. qRT-PCR was used to detect the mRNA expression levels of TXNIP, NLRP3, ASC, Caspase-1, interleukin-1β and interleukin-18. Western blot was used to detect the protein expression of TXNIP, Caspase-1 and NLRP3 in the cells. 
RESULTS AND CONCLUSION: Compared with the blank control group, intracellular reactive oxygen species and malondialdehyde levels were significantly increased in the model group, while the activity of superoxide dismutase and cell viability were significantly decreased. There was a significant increase in the secretion levels of interleukin-1β, interleukin-6, interleukin-18, tumor necrosis factor-ɑ as well as the gene expression levels of TXNIP, NLRP3, ASC, Caspase-1, interleukin-1β, interleukin-18 and the protein expression levels of TXNIP, Caspase-1 and NLRP3 in the model group compared with the blank control group. Baicalin could considerably reverse the above indicators in a concentration-dependent manner. To conclude, baicalin has a protective effect on the damage in BEAS-2B cells stimulated by lipopolysaccharide combined with adenosine triphosphate, by reducing oxidative stress and inhibiting the activation of the TXNIP/NLRP3 signaling pathway. 

Key words: baicalin, bronchus, epithelial cell, BEAS-2B cell, oxidative stress, inflammation, pathway, factor

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