Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (22): 3549-3555.doi: 10.3969/j.issn.2095-4344.1281
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Received:
2019-03-14
Contact:
Wang Yuze, MD, Associate chief physician, Department of Orthopedics, Second Hospital of Shanxi Medical University, Shanxi Provincial Key Laboratory of Bone and Soft Tissue Injury Repair, Taiyuan 030001, Shanxi Province, China
About author:
Yang Xuan, Master candidate, Attending physician, Department of Orthopedics, Second Hospital of Shanxi Medical University, Shanxi Provincial Key Laboratory of Bone and Soft Tissue Injury Repair, Taiyuan 030001, Shanxi Province, China
Supported by:
the National Natural Science Foundation of China, No. 31200728 (to WYZ); the Doctoral Startup Foundation of the Second Hospital of Shanxi Medical University, No. 20120408 (to WYZ)
CLC Number:
Yang Xuan, Han Pengfei, Zhou Xin, Lu Jiangong, Wang Shichuan, Wang Yuze. Protective effect of glucose-dependent incretin analogue DAIa2GIP on knee cartilage injury of rats[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(22): 3549-3555.
2.1 各组软骨形态大体观结果 见图1。 正常对照组软骨面光滑,有弹性,边缘整齐;模型对照组软骨表面粗糙,色泽灰暗,弹性差,软骨厚度变薄、软骨下骨外露现象明显,ICRS评分为3.80±0.79,明显低于正常对照组(P < 0.001);模型治疗组软骨欠光滑,有光泽,仍然可见少发裂纹,软骨层较模型对照组厚,未见骨外露现象,ICRS评分为7.10±1.20,明显高于模型对照组(P < 0.001);假手术对照组、假手术治疗组软骨表面呈胶冻样,光滑有弹性,边缘规整,未见纤维化及裂纹形成,ICRS评分分别为15.60±0.52,15.80±0.42,此两组评分与正常对照组比较无明显差异(P > 0.05),见图2。"
2.3 各组软骨番红O-固绿染色观察结果 见图4。 正常对照组蛋白多糖表达均匀,软骨厚度适中,Mankin评分为0.40±0.52;与正常对照组相比,模型对照组蛋白多糖表达明显不均匀,软骨厚度变薄,纤维化程度和范围重而且大,Mankin评分为12.90±0.74,评分低于正常对照组(P < 0.001);模型治疗组蛋白多糖表达基本均匀,软骨厚度较模型对照组增加,Mankin评分为7.30±1.16,评分低于模型对照组(P < 0.001);假手术对照组、假手术治疗组蛋白多糖表达均匀,软骨厚度正常,Mankin评分分别为0.60±0.70,0.80±0.789,此两组评分与正常对照组比较无明显差异(P > 0.05),见图5。"
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