Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (23): 3619-3624.doi: 10.12307/2021.029
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Liu Jinwei1, Chen Yunzhen2, Wan Chunyou1
Received:
2020-06-06
Revised:
2020-06-12
Accepted:
2020-07-14
Online:
2021-08-18
Published:
2021-01-26
Contact:
Wan Chunyou, Master, Chief physician, Department of Orthopedics, Tianjin Hospital, Tianjin 300211, China
About author:
Liu Jinwei, Master, Physician, Department of Orthopedics, Tianjin Hospital, Tianjin 300211, China
Supported by:
CLC Number:
Liu Jinwei, Chen Yunzhen, Wan Chunyou. Changes of osteogenic growth factors in the broken end of bone nonunion under stress[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(23): 3619-3624.
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实验组在第8周血管内皮生长因子出现高峰,与同组第4,12周比较差异有显著性意义(P < 0.05);第4周与第12周间表达无明显差异,但均高于同期对照组(P < 0.05,图7)。实验组转化生长因子β1在第4,8,12周与同期对照组比较差异有显著性意义(P < 0.05),实验组内比较差异无显著性意义(图8)。 对照组骨不连断端骨形态发生蛋白2、血管内皮生长因子、转化生长因子β1均有表达,但整体呈低表达,无峰值变化,组间的表达差异无显著性意义(P < 0.05)。 2.5 RT-PCR分析结果 实验组中骨形态发生蛋白基因表达在第8周出现峰值,与第4,12周比较差异有显著性意义(P < 0.05;图6),血管内皮生长因子基因扩增在第4周出现峰值,与第8,12周比较差异有显著性意义(P < 0.05;图7);转化生长因子β1在各期表达平稳,无高峰期,但表达量高于对照组,差异有显著性意义(P < 0.05;图8)。相对于对照组,实验组骨形态发生蛋白、血管内皮生长因子、转化生长因子β1基因扩增差异有显著性意义(P < 0.05)。"
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