Astragaloside IV suppresses the expression of inflammatory cytokines in chondrocytes via regulating NLRP3 inflammasome

doi:10.3969/j.issn.2095-4344.1068

Abstract

Abstract:

BACKGROUND: Astragaloside IV has been shown to hold a protective effect on arthritis, but its effect on inflammatory response in chondrocytes and the underlying mechanism are not yet clear.
OBJECTIVE: To investigate the effects of astragaloside IV on the activation of NLRP3 inflammasome and the releases of inflammatory cytokines in chondrocytes.
METHODS: Sprague-Dawely rat knee chondrocytes (Wuhan Bafeier Biological Co., Ltd.) were cultured in vitro, which were identified by type II collagen immunofluorescence. Chondrocytes were divided into five groups, including control group, lipopolysaccharide (1 mg/L)-induced group, and lipopolysaccharide plus low-dose (25 mmol/L), medium-dose (50 mmol/L), and high-dose (100 mmol/L) astragaloside IV groups. Cells were
treated with lipopolysaccharide and astragaloside IV for 24 hours. The relative cell survival rate was analyzed by cell count. The levels of interleukin-1β and tumor necrosis factor-α were detected by ELISA. The protein expression levels of NLRP3, caspase-1, interleukin-1β, and tumor necrosis factor-α were detected by western blot assay.
RESULTS AND CONCLUSION: The cell nucleus of chondrocytes was blue and cytoplasm was red under the fluorescent microscope. Compared with lipopolysaccharide-induced group, the relative survival rate of chondrocytes in each astragaloside IV group was significantly increased (P < 0.05). The levels of interleukin-1β and tumor necrosis factor-α in supernatant were significantly decreased (P < 0.05). The expression levels of NLRP3 inflammasome, caspase-1 and interleukin-1β and tumor necrosis factor-α were significantly decreased in a dose-dependent manner (P < 0.05). These results indicate that astragaloside IV can inhibit the expression of interleukin-1β and tumor necrosis factor-α protein in chondrocytes through inhibiting the activation of NLRP3 inflammasome, and further inhibit the inflammatory reaction of articular chondrocytes.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tissue Engineering, Chondrocytes, Lipopolysaccharides

CLC Number:

R453
R364

Yu Sujiao, Tan Hui. Astragaloside IV suppresses the expression of inflammatory cytokines in chondrocytes via regulating NLRP3 inflammasome [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(11): 1652-1656.

Figures/Tables 2

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