Insulin-like growth factor binding protein-2 promotes the proliferation, migration and invasion of glioma stem cells

doi:10.3969/j.issn.2095-4344.0999

Abstract

Abstract:

BACKGROUND: To date, the molecular mechanism of recombinant insulin-like growth factor binding protein 2 (IGFBP-2) on glioma is still unclear. Endogenous IGFBP-2 cannot explain a series of roles of IGFBP-2 in serum, and the roles of exogenous IGFBP-2 should be further elucidated.
OBJECTIVE: To observe the effect of IGFBP-2 on the proliferation, migration and invasion of glioma stem cells.
METHODS: CD133⁺ glioma stem cells were isolated from U251 human glioma cells by immunomagnetic bead technique. CD133 and nestin expression was detected by immunofluorescence staining. CD133⁺ glioma stem cells were divided into two groups: the cells were treated with 500 μg/L IGFBP-2 for 48 hours as experimental group, and the cells without intervention of IGFBP-2 as control group. Cell counting kit-8 method was used to detect cell proliferation ability. Transwell chamber assay was used to detect cell migration and invasion ability. Western blot assay was used to detect the expression of PCNA, Aki67, ERK and p-ERK proteins.
RESULTS AND CONCLUSION: The cell absorbance of the experimental group was significantly higher than that of the control group at 2-5 days of culture (P < 0.05), and the expression of PCNA and Ki-67 in the experimental group was also significantly higher than that in the control group (P < 0.05). The number of migrated cells and invasive cells in the experimental group was significantly higher than that in the control group (P < 0.05). Although there was no difference in the expression of ERK protein between the two groups, the expression of p-ERK protein in the experimental group was significantly higher than that in the control group (P < 0.05). To conclude, IGFBP-2 can promote the proliferation, migration and invasion of glioma stem cells, in which the ERK pathway may play a certain role.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Neoplastic Stem Cells, Insulin-Like Growth Factor Binding Protein 2, Glioma, Cell Proliferation, Cell Movement, Tissue Engineering

CLC Number:

R394.2

Zhao Wei, Tang Hui, Shao Chuan, Qiao Fei. Insulin-like growth factor binding protein-2 promotes the proliferation, migration and invasion of glioma stem cells[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(29): 4631-4636.

Figures/Tables 1

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