Topical usage of nerve growth factor-insulin gel on deep II scald wound increases microvessel density and proliferating cell nuclear antigen expression

doi:10.3969/j.issn.2095-4344.0847

Abstract

Abstract:

BACKGROUND: Researches have shown that nerve growth factor can promote wound healing, but there are few reports on the topical application of nerve growth factor combined with medication on diabetic scald wound.

OBJECTIVE: To explore the changes of microvessel density and proliferating cell nuclear antigen expression after topical application of nerve growth factor-insulin gel on deep II scald wound in diabetic rats.

METHODS: Seventy-five Wistar rats were randomly divided into five groups. Deep II scald models were made on the back of rats in normal control group followed by topical application of Carbopol 980. Diabetic models and deep II scald models were both made in the remaining rats with an interval of 3 days. Then, the rats in the latter four groups were subjected to topical application of Carbopol 980 alone, or Carbopol 980 containing nerve growth factor, insulin and combination of nerve growth factor and insulin, respectively. The wound in each rat was redressed once a day, for 21 consecutive days. Wound healing rate, microvessel density and proliferating cell nuclear antigen expression in rats were detected at 3, 7, 11, 15, 21 days after scald.

RESULTS AND CONCLUSION: Wound healing rate, microvessel density and proliferating cell nuclear antigen expression were higher in the combination group than the other four groups at different time after scald (P < 0.05). Results from hematoxylin-eosin staining showed that different structure damages, angiectasis and inflammatory cell infiltration were observed in different groups at 1 day after scald. A small amount of new capillaries were visible in each group, especially in the combination group at 3 days after scald. The number of capillary vessels and inflammatory cells increased gradually at 7-11 days after scald. Marked re-epithelialization and regular arrangement of collagen fibers were observed in the combination group at 15 days after scald. Meanwhile, compared with the normal control group and model group, the number of new vessels was increased in the nerve growth factor and insulin groups, but collagen fibers arranged disorderly. The wound in the combination group was covered with epithelial cells and collagen fibers arranged orderly at 21 days after scald, while there are different levels of epithelization in the other four groups. Higher level of epithelization was found in the nerve growth factor and insulin groups than the normal control and model groups. To conclude, topical application of nerve growth factor-insulin gel on deep II scald wound can promote wound healing by increasing microvessel density and proliferating cell nuclear antigen expression.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Gels, Biological Dressings, Insulin, Nerve Growth Factor, Diabetes Mellitus

CLC Number:

R318

Xu Hai-tao, Wang Min, Xiang Xi-juan. Topical usage of nerve growth factor-insulin gel on deep II scald wound increases microvessel density and proliferating cell nuclear antigen expression[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(22): 3506-3512.

Figures/Tables 12

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