Oxidized dextran/aminated carboxymethyl chitosan two-component hydrogel adhesive based on Schiff base reaction

doi:10.3969/j.issn.2095-4344.0846

Abstract

Abstract:

BACKGROUND: Dual-crosslinked hydrogel adhesives based on Schiff base reaction have certain network structure, good biocompatibility and high bond strength, which have good application prospects in tissue engineering and clinical medicine.

OBJECTIVE: To prepare and characterize oxidized dextran/aminated carboxymethyl chitosan two-component hydrogel adhesives through a Schiff base reaction.

METHODS: Dextran was oxidized with sodium periodate and carboxymethyl chitosan was aminated with ethylenediamine. Oxidized dextran/aminated carboxymethyl chitosan hydrogel adhesives were prepared by Schiff base reaction at room temperature. The structures of oxidized dextran and aminated carboxymethyl chitosan were characterized by infrared absorption spectroscopy. The iodine residue of oxidized dextran was measured by spectrophotometer, and the ethylenediamine residue of aminated carboxymethyl chitosan was determined by gas chromatography. The lap-shear tensile load strength, T-peel tensile load strength and tensile strength of oxidized dextran/carboxymethyl chitosan and oxidized dextran/aminated carboxymethyl chitosan hydrogel adhesives were tested using a universal testing machine according to industry standards.

RESULTS AND CONCLUSION: (1) IR spectra showed that the peak intensity of oxidized dextran decreased compared with that of dextran, with a new absorption peak appearing at 1 733 cm^-1, corresponding to the hemiacetal structure. The carboxymethyl chitosan itself had broad peak at 3 358 cm^-1, corresponding to the stretching vibration of the amine and hydroxyl groups, and the absorption peak at 3 358 cm^-1 of aminated carboxymethyl chitosan increased significantly. (2) The oxidation degree of dextran was 73.42%, and the iodine residue was 138.58 μg/g. The amino group content of aminated carboxymethyl chitosan was 0.636 9 mmol/L and no ethylenediamine resided. (3) The lap-shear tensile load strength, T-peel tensile load strength and tensile strength of oxidized dextran/aminated carboxymethyl chitosan hydrogel adhesives increased by 47.48%, 17.54% and 76.42%, respectively, with a significant increase compared with oxidized dextran/ carboxymethyl chitosan hydrogel adhesives. These results show that the oxidized dextran/aminated carboxymethyl chitosan hydrogel adhesive has higher lap-shear tensile load strength, T-peel tensile load strength and tensile strength.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Glucans, Chitosan, Hydrogel, Tissue Engineering

CLC Number:

R318

Li Dan-dan, Mo Xiu-mei . Oxidized dextran/aminated carboxymethyl chitosan two-component hydrogel adhesive based on Schiff base reaction[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(22): 3527-3532.

Figures/Tables 8

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