Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (20): 3144-3149.doi: 10.3969/j.issn.2095-4344.0820

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Shaoyang Shenggu formula regulates the expression of collagen type II and matrix metalloproteinase 13 in degenerative cartilage cells of C518 rat’s knee joint

Guan Tai-yuan1, Qi Ji2, Zhang Lei1, 2, Fu Shi-jie1, Ping Rui-yue3, Wang Guo-you1, Ao Liang1, Wang Li-sheng1,
Xu Yan-xiao2   

  1. 1Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China; 3Guangzhou University of Chinese Medicine, Guangzhou 510403, Guangdong Province, China
  • Received:2017-11-23 Online:2018-07-18 Published:2018-07-18
  • Contact: Zhang Lei, M.D., Attending physician, Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • About author:Guan Tai-yuan, Master, Attending physician, Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:

    the Science and Technology Supporting Program of Sichuan Province, No. 2014SZ0185; the Science and Technology Program of Luzhou, No. 2013LZLY-K59

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Abstract:

BACKGROUND: Shaoyang Shenggu formula is an effective prescription for osteoarthritis, which probably works via regulating cartilage degeneration, but the mechanism of action still needs an in-depth study.
OBJECTIVE: To explore the effects of Shaoyang Shenggu formula on the expression of collagen type II and matrix metalloproteinase 13 (MMP-13) in degenerative cartilage cells of C518 rat’s knee joint.
METHODS: C518 degenerative cartilage cells were selected, and then observed through hematoxylin-eosin staining. Afterwards, the cells were treated with Shaoyang Shenggu formula, glucosamine hydrochloride and normal saline, respectively. The expression levels of collagen type II and MMP-13 were detected by RT-qPCR and western blot assay.
RESULTS AND CONCLUSION: Compared with normal saline, Shaoyang Shenggu formula and glucosamine hydrochloride significantly up-regulated the mRNA and protein expression levels of collagen type II, and significantly down-regulated the mRNA and protein expression levels of MMP-13. Moreover, the regulatory effects were more obvious in the group of Shaoyang Shenggu formula (P < 0.05). To conclude, Shaoyang Shenggu formula delays the articular cartilage degeneration of the rat knee through regulating the expression of collagen type II and MMP-13 in C518 degenerative cartilage cells.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Drugs, Chinese Herbal, Chondrocytes, Collagen Type II, Matrix Metalloproteinases, Tissue Engineering

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