Interleukin-17A facilitates optic nerve regeneration in a mouse model of optic nerve crush

doi:10.3969/j.issn.2095-4344.0807

Abstract

Abstract:

BACKGROUND: Interleukin-17A has been shown to play an important role in nerve regeneration following corneal injury.
OBJECTIVE: To investigate the effect of interleukin-17A on mouse nerve regeneration after optic nerve injury.
METHODS: The interleukin-17A-overexpressed adenovirus vector (AV-IL-17A-GFP) was constructed and purified. Adenoviruses with green fluorescent protein (AV-GFP) served as control group. PC12 cell lines were transfected with adenoviruses and the expression efficiency of IL-17A were detected by ELISA. The adenovirus was intravitreally injected into the mouse vitreous body, and the expression level of IL-17A in the optic nerve was determined by immunofluorescence. Afterwards, a mouse model of optic nerve injury was established to observe the nerve regeneration.
RESULTS AND CONCLUSION: ELISA results showed that IL-17A was highly expressed in the PC12 cell lines transfected with AV-IL-17A-GFP compared with the control group. Immunofluorescence results revealed that IL-17A was expressed in vivo successfully. There was a significant increase in the number of regenerated axons in the AV-IL-17A-GFP group compared with the control group (P < 0.05). To conclude, AV-IL-17A-GFP can be expressed in the mature neurons in vivo and promote the axon regeneration after optic nerve crush injury in mice.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Models, Animal, Interleukin-17, Adenoviridae, Nerve Regeneration, Tissue Engineering

CLC Number:

R318

Li Yan-fen, Ye Xiao-yang, Chen Xiao-fan, Zhang Wei . Interleukin-17A facilitates optic nerve regeneration in a mouse model of optic nerve crush[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(12): 1883-1888.

Figures/Tables 2

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