Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (8): 1178-1183.doi: 10.3969/j.issn.2095-4344.0804
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Zhou Zhi-wen, Ju Li, Lou Yue
Received:
2017-11-08
Online:
2018-03-18
Published:
2018-03-18
Contact:
Lou Yue, Professor, Department of Orthopedics, Children’s Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China
About author:
Zhou Zhi-wen, Master, Physician, Department of Orthopedics Nanjing Children’s Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China
CLC Number:
Zhou Zhi-wen, Ju Li, Lou Yue. Quantitative peptidomic analysis of the cartilage tissues of different ages[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(8): 1178-1183.
2.1 差异软骨多肽定量分析 两组软骨多肽通过液相色谱-串联质谱分析共有5 027个多肽被鉴定出来,来源于 1 869种大分子蛋白质。差异多肽有588个,来源于428种蛋白质。如表1所示,16个多肽在高年龄组中高表达(大于4倍),6个多肽在低年龄组中高表达(大于4倍)。所有22个高表达多肽具有统计学意义(P < 0.05)。 2.2 差异软骨多肽等电点和相对分子质量分析 首先分析了差异软骨多肽的相对分子质量(图1A)和等电点(图1B),从中可以看出一些分布的不同。并且通过相对分子质量和等电点之间的分布差异可以看出软骨多肽分布的主要区域(图1C)。根据先前的研究[19],由于仪器和数据处理的局限性,液相色谱-串联质谱鉴定的多肽相对分子质量小于3 000。很明显在酸性和碱性pH范围内所识别的多肽聚集在4个区域。 2.3 差异软骨多肽基因本体分析 对鉴定的软骨多肽进行基因本体分析,由P值和FDR共同决定是否有统计学意义。并对潜在的生理功能分类:分子功能(图2),细胞组成(图3),生物过程(图4)。"
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