Effect of acupuncture on the expression level of interleukin-6 in rats after a single eccentric exercise

doi:10.3969/j.issn.2095-4344.0540

Abstract

Abstract:

BACKGROUND: Interleukin-6, is a cytokine that has wide sources. Interleukin-6 increases in the skeletal muscle after exercise, which plays an important role in motor function recovery. Acupuncture at the skeletal muscle after exercise can improve the ultrastructure of skeletal mscle, alleviate injury and promote functional recovery. However, acupuncture effects on the interleukin-6 post exercise remains unknown.
OBJECTIVE: To explore the influence of acupuncture intervention on interleukin-6 in rats after eccentric exercise.
METHODS: Totally 114 male Sprague-Dawley rats were randomly divided into four groups: control group (n=6), exercise group (n=36), acupuncture group (n=36) and exercise plus acupuncture group (n=36). The rats in the exercise and exercise plus acupuncture groups were submitted to eccentric exercise on a downward treadmill at 16° decline. The rats in the acupuncture and exercise plus acupuncture groups were subjected to the oblique acupuncture at the triceps surae. Samples except the control group were collected at 0, 2, 24, 48, 72 and 120 hours after treatment (n=6 per group at each time point). Protein expression level of interleukin-6 in the soleus and rectus femoris was detected by western blot assay. The plasma level of interleukin-6 was determined by ELISA.
RESULTS AND CONCLUSION: In the acupuncture group, the protein expression level of interleukin-6 in the soleus and rectus femoris and plasma level of interleukin-6 showed no significant changes (P > 0.05). After eccentric exercise, the interleukin-6 levels in the soleus, rectus femoris and plasma were significantly increased, and the protein expression level of interleukin-6 did not differ significantly between soleus and rectus femoris (P > 0.05). Acupuncture intervention after exercise, the protein expression level of interleukin-6 in the soleus was significantly reduced (P < 0.05), while interleukin-6 levels in the rectus femoris and plasma showed no significant change (P > 0.05). These findings imply that acupuncture cannot markedly change the interleukin-6 level in normal rats. Eccentric exercise can lead skeletal muscle to create more interleukin-6, and acupuncture intervention after eccentric exercise can reduce the amount of interleukin-6 at peak phase in the needling part. But its effect is local, not significant on the skeletal muscle of other parts and plasma.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Muscle, Skeletal, Interleukin-6, Acupuncture, Tissue Engineering

CLC Number:

R318

Bai Sheng-chao1, Chen Sheng-ju1, Wang Bo1, Wu Ying1, Shang Hua-yu2. Effect of acupuncture on the expression level of interleukin-6 in rats after a single eccentric exercise[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(32): 5191-5196.

Figures/Tables 2

2.1 实验动物数量分析实验选用大鼠114只，分为4组，实验过程中无脱失，全部进入结果分析。 2.2 比目鱼肌中白细胞介素6的变化情况分组方式的主体效应检验(P < 0.01)，进行事后多重检验，结果表明，对目前的结果分析而言，尚不能认为安静对照组和单纯针刺组之间差异有显著性意义(P > 0.05)，但安静对照组和单纯运动组、运动针刺组相比差异有显著性意义(P < 0.01)，单纯针刺组和运动针刺组差异显著性显著性意义(P < 0.01)，单纯运动组和运动针刺组差异显著性意义(P < 0.05)，其中安静对照组和单纯运动组的均值差异最大。提示：运动后进行比目鱼肌的针刺对该部位白细胞介素6的表达具有明显作用。取材时相的主体效应检验(P < 0.01)。进一步的事后多重检验，结果显示，0 h与2，24，48，120 h，2 h与24，48，72，120 h等差异都有显著性意义(P < 0.05或P < 0.01)，两因素交互作用检验(P < 0.05)。实验结果的单因素方差分析结果见表1，相应的蛋白表达见图1。 2.3 股直肌中白细胞介素6的变化分组方式的主体效应检验(P < 0.01)，进行事后多重检验，结果表明，就目前的结果分析而言，尚不能认为安静对照组与单纯针刺组之间、单纯运动组与运动针刺组之间差异有显著性意义(P > 0.05)，但安静对照组和单纯运动组、运动针刺组相比差异有显著性意义(P < 0.01)，单纯针刺组和运动针刺组差异有显著性意义 (P < 0.01)，其中安静对照组和单纯运动组的均值差异最大。提示：运动后进行比目鱼肌针刺对股直肌中白细胞介素6表达并未有显著作用。取材时相的主体效应检验(P < 0.01)，进一步的事后多重检验，结果显示，0 h与2，24，48，120 h以及2 h、120 h与所有时相相比差异都有显著性意义(P < 0.05或P < 0.01)，两因素交互作用检验(P < 0.05)。实验结果的单因素方差分析结果见表2，相应的蛋白表达见图2。 2.4 离心运动后大鼠比目鱼肌与股直肌中白细胞介素6的比较对单纯运动组大鼠比目鱼肌与股直肌中两组白细胞介素6的蛋白表达情况进行统计学检验，就目前的结果分析而言尚不能认为差异有显著性意义(P > 0.05)。 2.5 大鼠血浆中白细胞介素6的变化分组方式的主体效应检验(P < 0.01)，进行事后多重检验，结果表明，就目前的结果分析而言，尚不能认为安静对照组与单纯针刺组之间、单纯运动组与运动针刺组之间差异有统计学意义，但安静对照组和单纯运动组、运动针刺组相比较差异均有显著性意义(P < 0.01)，单纯针刺组和运动针刺组两组相比差异也同样有显著性意义(P < 0.01)，其中安静对照组和单纯运动组的均值差异最大。提示：运动后进行比目鱼肌针刺对血浆中白细胞介素6质量浓度并未有显著作用。取材时相的主体效应检验(P < 0.01)，进一步的事后多重检验，结果显示，0 h与120 h，2h与24，48，72，120 h等差异均有显著性意义(P < 0.05)，两因素交互作用检验(P > 0.05)。实验结果的单因素方差分析结果见表3。"

References

[1] 方剑乔,邵晓梅.针刺镇痛的新思路——针灸参与疼痛多维度调节的可行性[J].针刺研究,2017,42(1):85-89.

[2] 严晓艺,贾丽燕,张云皎,等.针刺治疗脑卒中后肌痉挛的系统综述与Meta分析[J].北京中医药大学学报,2017,40(1):52-58.

[3] 高纺,盛红梅,张田宁,等.针刺不同穴方对便秘小鼠肠运动的影响[J].针刺研究,2017,42(1):62-66+75.

[4] 尚画雨,付玉,夏志,等.针刺对运动性骨骼肌损伤大鼠骨骼肌线粒体自噬的影响[J].中国病理生理杂志,2017,33 (11):2038-2046.

[5] 丁海丽. 运动及针刺干预对骨骼肌内质网应激—自噬反应的影响机制研究[D].北京:北京体育大学,2017.

[6] 刘阳,王瑞元,康红哲.不同时程下[Ca~(2+)]介导骨骼肌微细损伤与修复的机制及针刺的干预作用[J].北京体育大学学报, 2015,38(2): 54-60.

[7] 孙君志.离心运动诱导骨骼肌细胞微管损伤的机制及针刺干预研究[D].北京:北京体育大学,2014.

[8] 白胜超, 赵晓琴, 孙君志,等. 针刺对一次离心运动后大鼠炎症反应的影响[J].首都体育学院学报, 2017,29(1):85-90.

[9] Baeza-Raja B, Muñoz-Cánoves P.p38 MAPK-induced nuclear factor-kappaB activity is required for skeletal muscle differentiation: role of interleukin-6. Mol Biol Cell.2004;15(4): 2013-2026.

[10] 唐晖,谢敏豪.运动引起肌源性IL-6分泌的研究进展[J].中国运动医学杂志,2006,25(1):60-66.

[11] 王瑞元,周越.骨骼肌与运动[M].北京:人民体育出版社,2013:1-19.

[12] Armstrong RB, Ogilvie RW, Schwane JA. Eccentric exercise-induced injury to rat skeletal muscle J Appl Physiol Respir Environ Exerc Physiol.1983;54(1):80-93.

[13] Mcginnis GR, Ballmann C, Peters B, et al. Interleukin-6 mediates exercise preconditioning against myocardial ischemia reperfusion injury. Am J Physiol Heart Circ Physiol. 2015;308(11): H1423-1433.

[14] Ameis K,Trouth AJ,Bond V,et al.Degeneration/Regeneration as a Mechanism Contributing to the Effect of Manual Acupuncture- Induced Injury on Rat Skeletal Muscle.Intech,2011.

[15] Pedersen BK, Hoffman-Goetz L. Exercise and the immune system: regulation, integration, and adaptation. Physiol Rev.2000; 80(3):1055-1081.

[16] Ullum H, Haahr PM, Diamant M, et al. Bicycle exercise enhances plasma IL-6 but does not change IL-1 alpha, IL-1 beta, IL-6, or TNF-alpha pre-mRNA in BMNC.J Appl Physiol (1985).1994;77(1): 93-97.

[17] Steensberg A, van Hall G, Osada T, et al. Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6. J Physiol. 2000;529(Pt 1):237-242.

[18] 苏全生,田野,王东辉,等.大蒜素对大鼠离心运动后血IL-6、CK、CK-MM和Ca2+变化的影响[J].体育科学,2006,26(3):73-76.

[19] 赵广高,苏全生,张慧,等.大蒜素与联合抗氧化剂干预对运动员DOMS和血浆IL-6水平变化的影响[J].体育科学, 2008,28(7):66-71.

[20] Rohde T,MacLean DA,Richter EA,et al.Prolonged submaximal eccentric exercise is associated with increased levels of plasma IL-6.Am J Physiol.1997;273(1 Pt 1):E85-91.

[21] Ostrowski K, Rohde T, Zacho M, et al. Evidence that interleukin-6 is produced in human skeletal muscle during prolonged running.J Physiol.1998;508(Pt 3):949-953.

[22] Bruunsgaard H,Galbo H,Halkjaer-Kristensen J,et al.Exercise- induced increase in serum interleukin-6 in humans is related to muscle damage.J Physiol.1997;499(Pt 3):833-841.

[23] Hiscock N,Chan MH, Bisucci T, et al.Skeletal myocytes are a source of interleukin-6 mRNA expression and protein release during contraction: evidence of fiber type specificity.FASEB J. 2004;18(9):992-994.

[24] Fischer CP, Hiscock NJ, Penkowa M, et al. Supplementation with vitamins C and E inhibits the release of interleukin-6 from contracting human skeletal muscle.J Physiol.2004;558(Pt 2): 633-645.

[25] Pernille K, Milena P, Charlotte K, et al. Interleukin-6 receptor expression in contracting human skeletal muscle；regulating role of IL-6. FASEB J. 2005;19(9):1181-1183.

[26] 王今越,丁树哲,刘伟,等.运动与IL-6的研究进展[J].体育科学,2007, 27(6):60-69.

[27] 林玉敏.艾灸对慢性疲劳模型大鼠的免疫干预及临床研究[D].广州:广州中医药大学,2014.

[28] Hsu CP, Chung YC. Influence, of interleukin-6, on the invasiveness, of human, colorectal, carcinoma.Anticancer Research, 2016, 26(6B):4607-4614.

[29] Roxburgh CS, Mcmillan DC.Therapeutics targeting innate immune/inflammatory responses through the interleukin-6/JAK/ STAT signal transduction pathway in patients with cancer.Transl Res. 2016;167(1):61-66

[30] 郑盛,杨涓,陈文钦,等.人脐带间充质干细胞通过IL-6/STAT3信号通路促进肝癌HepG-2细胞的增殖[J].中国组织工程研究, 2016,20(50): 7460-7468.