Effect of the combination of aerobic exercise and flavonoids from Lycium ruthenicum Murr on the inflammatory cytokine levels in a mouse model of alcoholic liver injury

doi:10.3969/j.issn.2095-4344.0370

Abstract

Abstract:

BACKGROUND: There is still a lack of an effective treatment method for alcoholic liver injury. Extracts from Lycium ruthenicum Murr have been shown to play a role in immunoregulation, reducing blood lipid and glucose, antioxidation, prevention and treatment of cardiovascular disease and tumor.
OBJECTIVE: To investigate the effect of aerobic exercise combined with flavonoids from Lycium ruthenicum Murr on alcoholic liver injury and its anti-inflammatory mechanism.
METHODS: Forty healthy male Kunming mice were used to construct alcoholic liver injury models. The rat models were randomly divided into model, flavonoids, aerobic exercise and aerobic exercise + flavonoids from Lyceum ruthenicum Murr (combination) groups. Aerobic exercise and intragastrical administration of flavonoids from Lyceum ruthenicum Murr were given at 1 day after modeling for 6 weeks. Afterwards, the mice were fasted overnight and then the liver tissues were removed. Hematoxylin-eosin staining and histopathological scoring were conducted. The serum contents of glutamic pyruvic transaminase, glutamic oxaloacetic transaminase and total bilirubin were tested by biochemical analysis, and the mRNA expression levels of tumor necrosis factor α, nuclear factor κB, interleukin 6 and interleukin 10 in the liver tissues and blood were determined by real-time PCR.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining and histopathological changes: in the model group, the hepatic cord arranged in disorder, hepatic lobules were damaged severely, and there were abundant uneven fat vacuoles and inflammatory cells in the cytoplasm. The damage degree of liver tissues was significantly lowered in the flavonoids and aerobic exercise groups (P < 0.05). The necrotic hepatocytes, fat vacuoles and inflammatory cell infiltration were significantly decreased in the combination group (P < 0.01). Compared with the model group, the serum contents of glutamic pyruvic transaminase, glutamic oxaloacetic transaminase and total bilirubin in the combination (P < 0.01) and aerobic exercise (P < 0.05) groups were significantly reduced. Real-time PCR results: compared with the model group, the mRNA expression levels of tumor necrosis factor α, nuclear factor κB, interleukin 6 in the liver tissues and blood in the three treatment groups were significantly reduced (P < 0.05), especially in the combination group (P < 0.01), and the mRNA expression level of interleukin 10 was significantly increased. Our findings manifest that aerobic exercise and flavonoids from Lycium ruthenicum Murr can effectively reduce the expression levels of inflammatory cytokines. As a minimally invasive early detection method, circulating RNA detection of plasma inflammatory factors is an important marker in the screen, observation and prognosis of alcoholic liver injury.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Hepatitis, Alcoholic, Lycium, Cytokines, Tissue Engineering

CLC Number:

R318

Li Di1, Chen Rui1, Chen Jia-qin1, Zheng He2, Zhou Bai-cun1, Mao Hai-feng1, 3, Chen Yi-lin1, Qu Hong-lin1, 3, Chen Wei1, Peng Qi1,Luo Chi-miao2. Effect of the combination of aerobic exercise and flavonoids from Lycium ruthenicum Murr on the inflammatory cytokine levels in a mouse model of alcoholic liver injury[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(32): 5197-5202.

Figures/Tables 2

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