Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (9): 1332-1337.doi: 10.3969/j.issn.2095-4344.0461

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Diazoxide effects on proliferation and apoptosis of bone marrow mesenchymal stem cells under hypoxic conditions

Li Hong-bo1, Lin Shi-wei1, Yao Jiang-ling2, Bian Yang-yang2, Qu Ye1, Wang Lei-tao2, Peng Lei2   

  1. 1The Second Affiliated Hospital of Hainan Medical University, Haikou 570102, Hainan Province, China; 2The First Affiliated Hospital of Hainan Medical University, Haikou 570102, Hainan Province, China
  • Revised:2017-12-21 Online:2018-03-28 Published:2018-04-03
  • Contact: Peng Lei, M.D., Chief physician, Professor, the First Affiliated Hospital of Hainan Medical University, Haikou 570102, Hainan Province, China
  • About author:Li Hong-bo, Master, Attending physician, the Second Affiliated Hospital of Hainan Medical University, Haikou 570102, Hainan Province, China. Lin Shi-wei, Chief physician, the Second Affiliated Hospital of Hainan Medical University, Haikou 570102, Hainan Province, China. Li Hongbo and Lin Shi-wei contributed equally to this work.
  • Supported by:

    the National Natural Science Foundation of China, No. 81460339; the Natural Science Foundation of Hainan Province, No. 30863; the Culture Fund of Hainan Medical University, No. HY2018-22

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Abstract:

BACKGROUND: It is an urgent problem to effectively make bone marrow mesenchymal stem cells exert proper effects under hypoxic preconditioning.
OBJECTIVE: To investigate the effects of diazoxide, a Mito-KATP channel activator, on the proliferation and apoptosis of mouse BMSCs in hypoxic environment.
METHODS: Mouse BMSCs were divided into four groups: blank control group, 0.16, 0.8, 4 μmol/L diazoxide groups. Cells intervened by diazoxide were cultured in a 10% O2 incubator. MTT assay was performed to detect cell proliferation at 1, 2, 4, 6, 8 days after intervention, and Hoechst 33258 staining was performed to observe cell apoptosis at 14 days after intervention. 
RESULTS AND CONCLUSION: High homogeneity and purity but low proliferation of BMSCs was found. There was no significant difference in the activity of BMSCs among 0.16, 0.8, 4 μmol/L diazoxide groups (P > 0.05). In the blank control group, concentrated nuclei were dark blue in color and aggregated, and several round apoptotic bodies were found. In the diazoxide groups, apoptotic bodies were occasionally found, and no significant difference was found among different diazoxide groups. These findings indicate that a certain concentration of diazoxide can reduce cell apoptosis but has no effects on the proliferation of mouse BMSCs under hypoxic environment (10% O2).

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Cell Hypoxia, Diazoxide, Apoptosis, Cell Proliferation, Tissue Engineering

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