Gastrodin effects on the neural functional recovery and expression of glial fibrillary acidic protein in a rat model of spinal cord injury

doi:10.3969/j.issn.2095-4344.0090

Abstract

Abstract:

BACKGROUND: Gastrodin has been shown to inhibit the in vivo astrocyte activation after injury, improve the microenvironment of spinal cord injury (SCI), and reduce secondary injuries.
OBJECTIVE: To observe the effects of gastrodine on the neural functional recovery and glial fibrillary acidic protein in a rat model of clamping-induced SCI.
METHODS: Forty-eight adult Sprague-Dawley rats were randomly divided into three groups, and the models of clamping-induced SCI were established. The rats in sham group were only subjected to laminectomy; gastrodine group was given 100 mg/kg•d gastrodine; the sham and SCI groups were given equal volume of normal saline. The function recovery of spinal cord was evaluated by Basso, Beattie, Bresnahan (BBB) scores, and Rivlin inclined plane experiment at postoperative 1, 3, 7, 14, 21 and 28 days. The morphological changes of spinal cord were observed after perfusion at 28 days, expression level of glial fibrillary acidic protein was detected by immunofluorescence, and changes of Nissl body were observed through Nissl staining.
RESULTS AND CONCLUSION: BBB and Rivlin inclined plane scores in each group were significantly decreased postoperatively, and the scores then increased with time; the scores at each time point in the sham group were significantly higher than those in the other groups (P < 0.05); the scores in the gastrodine group were significantly higher than those in the SCI group from day 3 (P < 0.05). At postoperative 28 days, the expression level of glial fibrillary acidic protein was the lowest in the sham group, followed by gastrodine group, and the highest in the SCI group (P < 0.05). In the gastrodine and SCI groups, Nissl bodies were slightly stained partially dissolved, and the account of Nissl bodies in this two groups was less than that in the sham group (P < 0.05), but the gastrodine group had significantly more Nissl bodies than the SCI group (P < 0.05). The spinal cord in the sham group revealed the complete structure; in the SCI group, the structure of spinal cord was in disorder, syringomyelia formatted, and the gray-white matter boundary was obscure; gastrodine group showed smaller syringomyelia and gray-white matter boundary were clear. These results show that gastrodin can inhibit the proliferation of astrocytes after SCI, play neuroprotection, and improve motor function.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Spinal Cord Injuries, Gastrodia, Nerve Regeneration, Tissue Engineering

CLC Number:

R318

Xiao Xue-fei1, 3, Li Juan-juan2, Huang Hui1, Zhang Xiang1, Huang Yao1, Li Jing-hui1, Yu Hua-lin1. Gastrodin effects on the neural functional recovery and expression of glial fibrillary acidic protein in a rat model of spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(4): 558-563.

Figures/Tables 2

2.1 实验动物数量分析实验选用大鼠48只，分为3组，实验过程中无脱失，全部进入结果分析。 2.2 脊髓损伤模型评价完成钳夹脊髓损伤的大鼠术后均出现损伤节段以下截瘫、血尿和尿潴留，术后因泌尿系感染及膀胱破裂死亡7只，其中天麻素组死亡3只，脊髓损伤组死亡4只，均用同体质量雄性SD大鼠补充。 2.3 行为学评定结果 BBB运动功能评分及Rivlin斜板实验各组大鼠术前差异无显著性意义(P > 0.05)。造模成功后分别于术后1，3，7，14，21，28 d进行BBB评分和斜板实验，统计分析结果。 2.3.1 BBB评分术后各组BBB评分均下降，随着时间推移各组评分逐渐升高，假手术组评分于各时相点均高于其余各组(P < 0.05)，于术后一二周运动功能恢复正常。术后3 d及其后各时间观察点，天麻素组评分均明显均高于脊髓损伤组，差异有显著性意义(P < 0.05)，见表1。 2.3.2 Rivlin斜板实验术后各组斜板实验结果数值均降低，随后逐渐恢复，假手术组斜板实验结果数值于各时相点均高于其余各组(P < 0.05)，于术后一二周运动功能恢复正常。术后3 d及其后各时间观察点，天麻素组斜板实验结果数值均明显均高于脊髓损伤组，差异有显著性意义(P < 0.05)，见表2。 2.4 脊髓组织病理变化假手术组脊髓结构完整，与周围组织结构清晰，天麻素组和脊髓损伤组脊髓与周围组织粘连明显，难以剥离，损伤处局部组织缺损，天麻素组脊髓粘连和组织缺损程度、范围明显较脊髓损伤组轻。苏木精-伊红染色结果：假手术组脊髓结构完整，灰白质界限清楚，灰质内神经元形态正常。脊髓损伤组脊髓组织结构排列混乱，灰白质界限不清，大量星形胶质细胞增生，脊髓空洞形成。天麻素组脊髓空洞较脊髓损伤组明显减小，灰白质结构尚清，灰质内见少量神经元，见图1。 2.5 脊髓胶质纤维酸性蛋白表达情况术后4周，低倍镜下脊髓损伤组和天麻素组脊髓纵切面见钳夹部位周围大量绿色荧光的胶质纤维酸性蛋白阳性细胞，胞体呈星形，向周围发出许多长而分支的突起，损伤边缘组织胶质纤维酸性蛋白表达最为强烈，出现明显胶质界膜。假手术组、脊髓损伤组和天麻素组的平均吸光度总和分别是 9 036.09±144.03，21 516.6±972.33，15 067.44±909.12，"

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