Preparation and performance properties of functionalized multi-walled carbon nanotubes/chitosan-zinc composite scaffolds

doi:10.3969/j.issn.2095-4344.0064

Abstract

Abstract:

BACKGROUND: A pure chitosan (CS) scaffold has poor mechanical properties, and cannot play a load-bearing role in the bone defect region. Therefore, many scholars attempt to develop multi-walled carbon nanotube (MWCNT)/chitosan (MWCNT/CS) bone tissue scaffolds.

OBJECTIVE: To develop the functionalized MWCNT/CS composite scaffolds carrying zinc (f-MWCNT/CS-Zn) and to test its mechanical properties, antibacterial activity and biocompatibility.

METHODS: (1) F-MWCNT composite scaffolds were prepared by the acid mixture method, and f-MWCNT/CS-Zn composite scaffolds were prepared through mixing CS, f-MWCNT (0.5%) with different concentrations of ZnSO₄•7H₂O (the concentrations of Zn in the composite scaffolds were 0.2%, 1%, 2%, respectively). The surface hardness and tensile strength of the pure CS scaffold and the f-MWCNT/CS-Zn composite scaffolds were measured. (2) The inhibitory effect on Staphylococcus aureus or Escherichia coli of the pure CS scaffold and f-MWCNT/CS-Zn scaffolds was tested by inhibition zone test. (3) The osteoblasts MC3T3-El were cultured with the leaching solution of f-MWCNT/CS-Zn composite scaffolds. The cell proliferation was detected by MTT assay at 1, 2, 3 days after culture, then the relative proliferation rate was calculated and the cytotoxicity was evaluated.

RESULTS AND CONCLUSION: (1) The surface hardness and tensile strength of the f-MWCNT/CS-Zn composite scaffolds were higher than those of the pure CS scaffold (P < 0.05), and there was no significant difference in the surface hardness and tensile strength between the different zinc-containing composite scaffolds. (2) The antibacterial properties of the three f-MWCNT/CS-Zn composite scaffolds against Staphylococcus aureus and Escherichia coli were higher than those of the pure CS scaffold (P < 0.05), and the antibacterial activity was enhanced with the increase of zinc mass fraction. (3) The f-MWCNT/CS-0.2%Zn and f-MWCNT/CS-1%Zn composite scaffolds had no cytotoxicity. To conclude, the f-MWCNT/CS-1%Zn composite scaffold has good mechanical properties, antibacterial activity and biocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Chitosan, Nanotubes, Carbon, Zinc, Tissue Engineering

CLC Number:

R318

Yang Ben, He Dong-ning, Qin Bo-heng, Zhang Bao-ping. Preparation and performance properties of functionalized multi-walled carbon nanotubes/chitosan-zinc composite scaffolds[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(6): 889-895.

Figures/Tables 7

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