Injectable nano-hydroxyapatite/chitosan composite scaffolds combined with bone marrow mesenchymal stem cells and bone morphogenetic protein 2 for bone defect repair in vitro

doi:10.3969/j.issn.2095-4344.0011

Abstract

Abstract:

BACKGROUND: Bone morphogenetic protein 2 (BMP-2) has a strong ability to induce and promote the osteogenic differentiation of mesenchymal stem cells.

OBJECTIVE: To evaluate the BMP-2 effect on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) on an injectable nano-hydroxyapatite/chitosan (nHA/CS) composite scaffold.

METHODS: (1) Experiment 1: Passage 3 BMSCs were divided into two groups and cultured with the nHA/CS scaffold or cultured alone. Cell counting kit-8 was used to detect cell proliferation at 1, 3, 5, 7, 14 days of culture. (2) Experiment 2: Passage 3 BMSCs were seeded onto the nHA/CS scaffold and cultured in culture medium containing BMP-2 or not. Alkaline phosphatase activity in cells was detected at 3, 6, 9, 12, 15 days of culture. Cell counting kit-8 was used to detect cell proliferation at 1, 3, 5, 7, 14 days of culture. Alizarin red staining was used to observe the osteogenic differentiation of cells at 1 and 2 weeks of culture.

RESULTS AND CONCLUSION: (1) Experiment 1: With the prolongation of culture time, the absorbance values in the two groups were gradually increased, but there was no significant difference between the two groups. At 7 days of culture, the BMSCs adhered tightly to the scaffold surface. (2) Experiment 2: With the prolongation of culture time, the alkaline phosphatase activities in the two groups were gradually increased, and moreover, the alkaline phosphatase activity in the experimental group was higher than that in the control group at different culture time (P < 0.05). The absorbance values in the two groups were also gradually increased, and the value in the experimental group was higher than that in the control group at different culture time (P < 0.05). At 1 and 2 weeks of culture, the number of calcified nodules was higher in the experimental group than the control group. To conclude, BMP-2 has a promotion role in the proliferation and differentiation of BMSCs cultured on the injectable nHA/CS scaffold.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Bone Morphogenetic Proteins, Hydroxyapatites, Chitosan, Cell Proliferation, Tissue Engineering

CLC Number:

R318

Liu Guang-tao, Gao Feng, Xu Jun, Zheng Wei-zhuo, Lin Xiao-zong, Zhou Chang-lin, Guo Ya-shan, Tian Jun.

Injectable nano-hydroxyapatite/chitosan composite scaffolds combined with bone marrow mesenchymal stem cells and bone morphogenetic protein 2 for bone defect repair in vitro

[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(2): 228-233.

Figures/Tables 8

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