Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (25): 4591-4596.doi: 10.3969/j.issn.1673-8225.2011.25.009

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Acellular nerve scaffold loading bone marrow mesenchymal stem cells to prepare artificial nerve for sciatic nerve defects

Zhang Cai-shun, Lü Gan   

  1. Department of Hand and Foot Surgery, First Affiliated Hospital of Liaoning Medical University, Jinzhou  121001, Liaoning Province, China
  • Received:2011-03-05 Revised:2011-04-26 Online:2011-06-18 Published:2014-01-10
  • Contact: Lü Gang, Professor, Doctoral supervisor, Department of Hand and Foot Surgery, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • About author:Zhang Cai-shun☆, Doctor, Associate professor, Department of Hand and Foot Surgery, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China zcs731009@163.com
  • Supported by:

    Innovative Team Project of Higher Education, Liaoning Education Bureau, No. 2008T114*

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Abstract:

BACKGROUND: The authors have successfully prepared an acellular nerve graft to be combined with bone marrow mesenchymal stem cells in artificial nerve bridge of rat sciatic nerve defects.
OBJECTIVE: To investigate the motor function of rats with sciatic nerve defects following repairing using acellular nerve graft combined with bone marrow mesenchymal stem cells.
METHODS: Adult SD rats were used to prepare rat models of 15-mm sciatic nerve defects. Tissue-engineered nerve, tissue-engineered nerve scaffold, and autogenous nerve were separately applied to bridge sciatic nerve defects. After 20 weeks, electrophysiological determination of regenerated nerves was conducted, wet weight of the anterior tibial muscle was determined, and histological and transmission electron microscopy analyses of the gastrocnemius was done.
RESULTS AND CONCLUSION: Twenty weeks after nerve grafting, no difference in the wet weight of the anterior tibial muscle was shown to be significant between tissue-engineered nerve and autogenous nerve grafts (P > 0.05). The nerve conduction velocity was (30.56±2.15) m/s. The findings indicate that the acellular nerve graft combined with bone marrow mesenchymal stem cells can promote the recovery of motor function in rats with sciatic nerve defects.

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