Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (32): 5097-5103.doi: 10.12307/2024.496
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Liu Xueli1, Shen Li1, Bi Wenguang1, Mou Yang1, Li Sen2
Received:
2023-08-16
Accepted:
2023-09-25
Online:
2024-11-18
Published:
2023-12-28
Contact:
Li Sen, MD, Chief physician, Department of Spine Surgery, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medicine School, Nanjing 210000, Jiangsu Province, China
About author:
Liu Xueli, Master, Rehabilitation therapist, School of Rehabilitation, Sichuan Vocational College of Health and Rehabilitation, Zigong 643000, Sichuan Province, China
Supported by:
CLC Number:
Liu Xueli, Shen Li, Bi Wenguang, Mou Yang, Li Sen. Effect and mechanism of low intensity pulsed ultrasound on early angiogenesis in rats with acute tendon injury[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(32): 5097-5103.
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正常组肌腱组织(图2C)胶原纤维排列平整紧密,肌腱细胞呈纺锤形并平行于纤维,腱周组织少且几乎无血管,其他各组肌腱组织(图2A,B,D,E)均有不同程度的细胞增多、炎性细胞浸润、胶原纤维排列紊乱和血管分布改变。参照国际Bonar评分量表对血管生成数量进行半定量分析,结果显示:与对照组相比,超声组的新生血管数量较同期增多,其中超声组2的新生血管数量显著高于对照组2,差异有显著性意义(P < 0.05);而超声组1与对照组1之间差异无显著性意义(P > 0.05,图2F)。 2.4 免疫组织化学检测血管标志物CD31的蛋白表达水平 各组大鼠跟腱组织免疫组化结果见图3,血管标记物CD31主要分布于腱周的血管内皮组织,部分分布于腱内外膜和肌腱细胞。正常组跟腱组织(图3C)几乎无CD31的阳性表达,对照组和超声组均表现不同程度的CD31蛋白阳性表达增加。与对照组相比,超声组跟腱组织中CD31阳性表达增加,并随着时间呈上升趋势,可见超声组2新生血管数量增多最为明显。"
2.5 Western blot检测大鼠跟腱组织中VEGF、YAP的蛋白表达 Western blot检测结果见图4。与正常组相比,经Ⅰ型胶原酶造模的各组受损肌腱中的VEGF、YAP蛋白表达量均有不同程度的增加。其中,超声组的VEGF蛋白表达量均高于同期对照组,但超声组1与对照组1相比,VEGF蛋白表达量无显著性差异(P > 0.05),超声组2与对照组2和正常组相比均明显升高,差异有显著性意义(P < 0.05)。此外,超声组和对照组随时间均有上升趋势,2周时VEGF蛋白表达水平高于1周时,但差异无显著性意义(P > 0.05)。检测Hippo信号通路中关键效应因子YAP蛋白表达水平发现,超声组2明显高于对照组2,差异有显著性意义(P < 0.05);超声组1高于对照组1,但差异无显著性意义(P > 0.05)。"
检测Hippo信号通路中关键效应因子YAP的mRNA表达量,发现造模后所有组的YAP基因表达量相比于正常组均有增加。各组间变化与VEGF表达趋势一致,超声组2的YAP基因表达量显著高于对照组2,差异有显著性意义(P < 0.05)。对YAP下游的Ang-2和CYR61的mRNA表达量进行统计,与同期对照组相比,超声组均有增加,但仅超声组2与对照组2之间差异有显著性意义(P < 0.05)。 对比超声组和对照组自身随时间的变化,结果显示随着肌腱愈合过程,超声组2和对照组2的VEGF、YAP、Ang-2和CYR61的mRNA表达量较超声组1和对照组1均有上升趋势,但差异均无显著性意义(P > 0.05)。 与其他因子mRNA表达量不同的是,LIPUS干预1周后超声组1的HIF-1α mRNA表达量相比于对照组1显著上升,差异有显著性意义(P < 0.05);然而,LIPUS干预2周后超声组2的HIF-1α mRNA表达量相比于对照组2显著降低,且差异有显著性意义(P < 0.05);对照组2的HIF-1α mRNA表达量相比于对照组1显著增加(P < 0.05),而超声组2相比于超声组1显著降低(P < 0.05)。"
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