Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (28): 4469-4476.doi: 10.12307/2024.340
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Tian Yong1, 2, Zhou Ying1, Gu Yongxiang1, Yang Guohui3
Received:
2023-04-18
Accepted:
2023-06-01
Online:
2024-10-08
Published:
2023-11-27
Contact:
Yang Guohui, Professor, Chief physician, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
About author:
Tian Yong, Master candidate, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; Intensive Care Unit, Tongren City People’s Hospital, Tongren 554300, Guizhou Province, China
Supported by:
CLC Number:
Tian Yong, Zhou Ying, Gu Yongxiang, Yang Guohui. Metformin pretreatment induces cardiac autophagy to reduce myocardial injury in septic mice[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(28): 4469-4476.
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2.1 造模小鼠的一般情况分析 假手术组10 只小鼠及CLP 6 h组10 只小鼠全部存活,假手术组小鼠术后24 h精神、活动及进食正常,无腹泻、畏寒、排泄物异常增多等情况;CLP 6 h组小鼠精神稍萎靡,活动基本正常,无腹泻;CLP 12 h组在术后12 h内死亡1 只小鼠,余存活9 只小鼠出现畏寒、腹泻,进食明显减少,活动明显减少,精神萎靡;CLP 24 h组死亡4 只小鼠,存活6 只小鼠出现嗜睡、拒食、腹泻、竖毛、呼吸急促、眼部有较多分泌物、运动迟缓、不能直立。 2.2 造模小鼠炎症因子及心肌损伤标志物的变化 ELISA检测各组小鼠血清TNF-α、IL-1β、CK-MB及cTnT水平,如图1所示,与假手术组相比,CLP各组炎症因子TNF-α、IL-1β水平均升高(P < 0.05),以CLP 24 h组最为显著;与假手术组相比,CLP 12 h组和CLP 24 h组CK-MB、cTnT水平均显著升高(P < 0.05),以CLP 24 h组更显著,而CLP 6 h组与假手术组相比无统计学差异(P > 0.05),表明在CLP 12 h脓毒症小鼠心肌损伤模型构建成功,且CLP 24 h组较CLP 12 h组心肌损伤更重。"
2.3 造模小鼠心肌组织中自噬水平 Western blot检测各组造模小鼠心肌组织自噬标志物(LC3B、p62)的表达水平,如图2所示,与假手术组相比,CLP 6 h组LC3Ⅱ/LC3Ⅰ显著升高(P < 0.05),之后逐渐降低,在CLP 24 h降至基础水平;与假手术组相比,CLP 6 h组p62表达水平显著降低(P < 0.05),之后逐渐恢复,在CLP 24 h已恢复至基础水平,CLP 24 h组与假手术组相比无统计学差异(P > 0.05),这提示在脓毒症早期,自噬处于高表达水平,但在脓毒症心肌损伤中自噬处于抑制状态,在CLP 24 h脓毒症小鼠中自噬处于基础水平,且心肌损伤较CLP 12 h严重,故此研究选择脓毒症心肌损伤中自噬处于显著抑制的时间点(24 h)进行后续研究。"
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