Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (7): 1029-1035.doi: 10.12307/2024.110
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Li Longyang, Zhang Songjiang, Zhao Xianmin, Zhou Chunguang, Gao Jianfeng
Received:
2023-01-06
Accepted:
2023-02-14
Online:
2024-03-08
Published:
2023-07-15
Contact:
Gao Jianfeng, Professor, Master’s supervisor, School of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, Henan Province, China
About author:
Li Longyang, Master candidate, School of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, Henan Province, China
Zhang Songjiang, Associate professor, Master’s supervisor, School of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, Henan Province, China
Supported by:
CLC Number:
Li Longyang, Zhang Songjiang, Zhao Xianmin, Zhou Chunguang, Gao Jianfeng. Electroacupuncture intervention on the proliferation and differentiation of hippocampal neurons and oligodendrocytes in Alzheimer’s disease model mice[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(7): 1029-1035.
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2.1 实验动物数量分析 实验选用雄性C57BL/6J小鼠20只,作为正常对照组,选用阿尔茨海默病模型小鼠40只,随机分为阿尔茨海默病模型组和电针穴位组,每组20只。实验过程中无小鼠死亡,所有小鼠全部进入结果分析。 2.2 各组小鼠Morris水迷宫检测结果 采用Morris水迷宫检测各组小鼠的学习和记忆功能。从图2可以看出,与正常对照组相比,模型组定位航行实验第2,3,4天潜伏期明显延长(P < 0.05),穿越平台次数(P < 0.05)和平台滞留时间明显减少(P < 0.05);与模型组相比,电针穴位组小鼠定位航行实验第3,4天潜伏期明显缩短(P < 0.05),穿越平台次(P < 0.05)和平台滞留时间明显增加(P < 0.05)。同时,随着时间的推移,不论是模型组与正常对照组相比,还是电针穴位组与模型组相比,潜伏期的变化越来越大,到第4天最大。"
2.4 各组小鼠海马齿状回BrdU/NeuN和BrdU/GALC免疫荧光双标结果 BrdU/NeuN和BrdU/GALC免疫荧光双标检测各组小鼠海马齿状回神经元和少突胶质细胞的增殖情况。从图4可以看出,与正常对照组相比,模型组小鼠海马齿状回BrdU/NeuN的阳性细胞表达没有明显变化(P > 0.05);与模型组相比,电针穴位组小鼠海马齿状回BrdU/NeuN的阳性细胞表达明显增加(P < 0.01)。从图5可以看出,与正常对照组相比,模型组小鼠海马齿状回BrdU/GALC的阳性细胞表达明显减少(P < 0.01);与模型组相比,电针穴位组小鼠海马齿状回BrdU/GALC的阳性细胞表达明显增多(P < 0.01)。"
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