Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (26): 4127-4135.doi: 10.12307/2022.814
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Zeng Chunrong, Liu Menglan, Xie Yang, Li Zuoxiao
Received:
2021-03-26
Accepted:
2021-05-12
Online:
2022-09-18
Published:
2022-03-08
Contact:
Li Zuoxiao, Professor, Master’s supervisor, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
About author:
Zeng Chunrong, Master, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
Supported by:
CLC Number:
Zeng Chunrong, Liu Menglan, Xie Yang, Li Zuoxiao. Preventive and therapeutic effects of naringin on experimental autoimmune encephalomyelitis via regulating microglial polarization[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(26): 4127-4135.
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2.5 柚皮苷减少小胶质细胞激活 与空白对照组比较,EAE模型组红染的目的蛋白Iba-1表达增多,Merge图中Iba-1蛋白明显增多,呈聚集现象,脊髓组织Iba-1阳性表达区域的平均吸光度值增加(P < 0.01);与EAE模型组比较,柚皮苷各剂量组Iba-1蛋白表达减少,Merge图中Iba-1蛋白减少,Iba-1阳性表达区域的平均吸光度值减少(P < 0.01),柚皮苷各剂量组间Iba-1阳性表达区域的平均吸光度值差异均有显著性意义(P < 0.01或P < 0.05),见图4及表5。说明EAE小鼠发病高峰期小胶质细胞激活明显,柚皮苷干预后可减少小胶质细胞激活,且柚皮苷剂量越大,效果越明显。"
2.6 柚皮苷促进M1型小胶质细胞向M2型转化 与空白对照组比较,EAE模型组M1型标记物诱导型一氧化氮合酶、CD16明显增加(P < 0.01或P < 0.05),而M2型标记物Arg-1、CD206明显减少(P < 0.01或P < 0.05);与EAE模型组比较,柚皮苷各剂量组CD16、诱导型一氧化氮合酶减少(P < 0.01),Arg-1、CD206增加(P < 0.01);柚皮苷各剂量组间比较,差异均有显著性意义(P < 0.01或P < 0.05),见图5及表6。说明EAE小鼠脊髓组织中发病高峰期以M1型小胶质细胞浸润为主,柚皮苷可促进M1型小胶质细胞向M2型转化,且柚皮苷剂量越大,变化越明显。"
2.8 柚皮苷降低EAE模型中LC3表达 与空白对照组比较,EAE模型组红染的目的蛋白LC3表达增多,Merge图中LC3蛋白明显增多,呈聚集现象,脊髓组织LC3阳性表达区域的平均吸光度值增加(P < 0.01);与EAE模型组比较,柚皮苷各剂量组LC3表达减少,Merge图中LC3蛋白减少,LC3阳性表达区域的平均吸光度值减少(P < 0.01或P < 0.05);柚皮苷各剂量组间比较,除中、高剂量组间LC3阳性表达区域的平均吸光度值差异无显著性意义,其余组间差异均有显著性意义(P < 0.01或P < 0.05),见图7及表8。说明EAE小鼠发病高峰期存在自噬激活,柚皮苷干预后可下调自噬,且柚皮苷剂量越大,效果越明显。"
2.9 柚皮苷下调EAE模型中自噬通量 与空白对照组比较,EAE模型组脊髓组织LC3-Ⅱ/LC3-Ⅰ灰度值比值、Beclin1表达增加(P < 0.01),p62表达减少(P < 0.01);与EAE模型组比较,柚皮苷各剂量组LC3-Ⅱ/LC3-Ⅰ灰度值比值、Beclin1表达减少(P < 0.01或P < 0.05),p62表达增加(P < 0.01);柚皮苷各剂量组间比较,LC3-Ⅱ/LC3-Ⅰ灰度值比值、Beclin1及p62差异均有显著性意义(P < 0.01或P < 0.05),见图8及表9。说明EAE小鼠发病高峰期存在自噬通量增加,柚皮苷干预后可降低自噬通量,且柚皮苷剂量越大,作用越明显。"
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