Effect of xanthohumol on inflammatory factors and articular cartilage in a mouse mode of osteoarthritis

doi:10.12307/2022.110

Abstract

Abstract: BACKGROUND: Studies have shown that xanthohumol has antioxidant and anti-inflammatory properties; however, there is no study regarding the precise effect of xanthohumol on osteoarthritis.
OBJECTIVE: To investigate the role of xanthohumol in the occurrence and development of osteoarthritis in vivo and in vitro.
METHODS: (1) A mouse osteoarthritis model was constructed by interleukin 1β treatment, followed by intervention with 0-40 µmol/L xanthohumol. The level of chondrocyte inflammatory factors was detected by ELISA, and the chondrocyte synthesis and metabolism indicators were detected by RT-PCR. (2) Surgery for medial meniscus instability was used to construct the arthritis model in mice, followed by intervention with 50 mg/kg/d xanthohumol for 6 weeks. Histological staining was used to evaluate the severity of damage to the articular cartilage, and μCT scan was used to analyze the changes in subchondral bone mass in the knee joint.
RESULTS AND CONCLUSION: The cytokines, such as nitric oxide, prostaglandin E2, tumor necrosis factor α and interleukin 6, which were produced in response to interleukin 1β-induced chondrocyte inflammation, were inhibited by xanthohumol pretreatment. Xanthohumol prevented the degradation of the extracellular chondrocyte matrix caused by interleukin 1β by down-regulating metalloproteinase 13 matrix and by increasing the synthesis of type II collagen. Mice treated with xanthohumol dramatically postponed the development of osteoarthritis caused by surgery, and had better articular cartilage morphology and maintained subchondral bone density. Overall, these findings indicate that xanthohumol can inhibit the synthesis of chondrocyte inflammatory mediators, inhibit the degradation of the extracellular matrix of the cartilage, and maintain the metabolic equilibrium in the articular cartilage.

Key words: knee osteoarthritis, articular cartilage, chondrocyte, inflammatory reaction, xanthohumol, mouse

CLC Number:

Lin Xuchen, Zhu Hainian, Wang Zengshun, Qi Tengmin, Liu Limin, Suonan Angxiu. Effect of xanthohumol on inflammatory factors and articular cartilage in a mouse mode of osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(5): 676-681.

Figures/Tables 4

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