Changes of type I and III collagen and matrix metalloproteinase 2 and 9 on the wound of diabetic foot ulcer with external application of medical collagen dressing

doi:10.12307/2022.201

Abstract

Abstract: BACKGROUND: Collagen has been widely used in the fields of oral cavity, blood vessel, nerve, bone and cartilage, but the application of collagen sponge in ulcer wound of diabetic foot is seldom studied.
OBJECTIVE: To analyze the effect of medical collagen sponge on the expression of type I and III collagen and matrix metalloproteinase 2 and 9 in granulation tissue of diabetic foot ulcer wound.
METHODS: Forty-six patients with diabetic foot ulcers, 25 males and 21 females, admitted to the Department of Podiatry, Metabolic Disease Hospital of Tianjin Medical University from January to December 2019 were randomly divided into two groups. The control group received dressing change and debridement. The experimental group was treated with collagen sponge on the basis of the control group. Wound area was recorded before treatment and 3, 7, 14 days after treatment. Wound healing rate was calculated. Granulation tissue was taken and immunohistochemical staining was used to observe the expression of matrix metalloproteinase 2 and matrix metalloproteinase 9. Western blot analysis was used to measure type I and III collagen protein expression. This study was approved by the Ethics Committee of Zhu Xianyi Memorial Hospital of Tianjin Medical University.
RESULTS AND CONCLUSION: (1) The wound healing rate of the two groups increased with the time of treatment. The wound healing rate of the test group was higher than that of the control group at 3, 7, and 14 days after treatment (P < 0.05). (2) With the treatment time prolonged, the deposition of matrix metalloproteinase 2 and matrix metalloproteinase 9 in wound granulation tissue decreased in both groups. The expression of matrix metalloproteinase 2 and 9 in the experimental group was lower than that in the control group at various time points (P < 0.05). (3) The expression of type I and type III collagen in the wound granulation tissue increased with the treatment time, and the expression of type I and type III collagen in the experimental group was higher than that in the control group (P < 0.05). (4) The results showed that medical collagen sponge could reduce the expression of matrix metalloproteinase 2/9 in wound tissue, promote the synthesis of type I and III collagen, and improve the wound healing rate.

Key words: material, collagen, diabetic foot ulcer, wound healing, matrix metalloproteinase, extracellular matrix

CLC Number:

Zhao Zixi, Xu Jun, Ding Min, Li Xiwen, Zhang Jinghang, Wang Penghua. Changes of type I and III collagen and matrix metalloproteinase 2 and 9 on the wound of diabetic foot ulcer with external application of medical collagen dressing[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(10): 1544-1550.

Figures/Tables 10

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