Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (3): 339-345.doi: 10.12307/2023.025
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Zhang Hui1, Wang Jiayang2, Wang Qian3, 4, Gan Hongquan2, Wang Zhiqiang2
Received:
2021-09-22
Accepted:
2022-01-13
Online:
2023-01-28
Published:
2022-05-19
Contact:
Wang Qian, MD, Associate professor, Master’s supervisor, School of Clinical Medicine, North China University of Science and Technology, Tangshan 063000, Hebei Province, China; Hebei Key Laboratory of Medical Engineering Integration Precision Medicine, Tangshan 063000, Hebei Province, China
About author:
Zhang Hui, MD, Associate chief physician, First Department of Joint Surgery, Second Hospital of Tangshan, Tangshan 063000, Hebei Province, China
Supported by:
CLC Number:
Zhang Hui, Wang Jiayang, Wang Qian, Gan Hongquan, Wang Zhiqiang. Effects of hyaluronic acid combined with domestic porous tantalum on chondrocyte function under the dynamic environment[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(3): 339-345.
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静态与动态培养环境下的蛋白聚糖、Ⅱ型胶原分泌量比较差异均有显著性意义(F=1041,P=0.000;F=76.221,P=0.000),B组蛋白聚糖、Ⅱ型胶原分泌量低于D组(P < 0.05),C组蛋白聚糖、Ⅱ型胶原分泌量低于E组(P < 0.05);静态与动态培养环境下的SOX9分泌量比较差异无显著性意义(F=0.023,P=0.883)。 透明质酸对3因子影响明显,透明质酸复合前后蛋白聚糖(F=92.881,P=0.000)、Ⅱ型胶原(F=9.565,P=0.015)、SOX9(F=6.721,P=0.032)分泌量比较差异均有显著性意义(P < 0.05)。 2.6 各组软骨细胞蛋白聚糖、Ⅱ型胶原、SOX9蛋白表达量 见图7。 "
静态与动态培养环境下的蛋白聚糖、Ⅱ型胶原蛋白表达量比较差异均有显著性意义(F=9.045,P=0.017;F=392.689,P=0.000),D组蛋白聚糖、Ⅱ型胶原蛋白表达量高于B组(P < 0.05),E组蛋白聚糖、Ⅱ型胶原蛋白表达量高于C组(P < 0.05);静态与动态培养环境下的SOX9蛋白表达量比较差异无显著性意义(F=0.331,P=0.581)。 透明质酸对3因子蛋白表达量影响明显,透明质酸复合前后蛋白聚糖(F=6.683,P=0.032)、Ⅱ型胶原(F=17.422,P=0.003)、SOX9(F=5.721,P=0.043)蛋白表达量比较差异均有显著性意义(P < 0.05)。 "
[1] HUBBARD MJ. Articular debridement versus washout for degeneration of the medial femoral condyle. A five-year study. Bone Joint Surg Br. 1996;78(2):217-219. [2] JACKSON RW, DIETERICHS C. The results of arthroscopic lavage and debridement of osteoarthritic knees based on the severity of degeneration: a 4- to 6-year symptomatic follow-up. Arthroscopy. 2003;19(1):13-20. [3] PAP T, KORB-PAP A. Cartilage damage in osteoarthritis and rheumatoid arthritis-two unequal siblings. Nat Rev Rheumatol. 2015;11(10): 606-615. [4] CHIANG H, KUO TF, TSAI CC, et al. Repair of porcine articular cartilage defect with autologous chondrocyte transplantation. J Orthop Res. 2005;23(3):584-593. [5] JAMIL K, CHUA KH, JOUDI S, et al. Development of a cartilage composite utilizing porous tantalum, fibrin, and rabbit chondrocytes for treatment of cartilage defect. J Orthop Surg Res. 2015;7(10):27. [6] YAZDIMAMAGHANI M, RAZAVI M, VASHAEE D, et al. Porous magnesium-based scaffolds for tissue engineering. Mater Sci Eng C Mater Biol Appl. 2017;1(71):1253-1266. [7] LIU Y, SU X, ZHOU S, et al. A modified porous tantalum implant technique for osteonecrosis of the femoral head: survivorship analysis and prognostic factors for radiographic progression and conversion to total hip arthroplasty. Int J Clin Exp Med. 2015;8(2):1918-1930. [8] KHAN FA, ROSE PS, YANAGISAWA M, et al. Surgical Technique: Porous Tantalum Reconstruction for Destructive Nonprimary Periacetabular Tumors. Clin Orthop Relat Res. 2012;470(2):594-601. [9] PATIL N, LEE K, GOODMAN SB. Porous Tantalum in Hip and Knee Reconstructive Surgery. J Biomed Mater Res B Appl Biomater. 2009; 89(1):242-251. [10] SALWOWSKA NM, BEBENEK KA, ŻĄDŁO DA, et al. Physiochemical properties and application of hyaluronic acid: a systematic review. J Cosmet Dermatol. 2016;15(4):520-526. [11] OZKAN FU, OZKAN K, RAMADAN S, et al. Chondroprotective effect of N-acetylglucosamine and hyaluronate in early stages of osteoarthritis-an experimental study in rabbits. Bull NYU Hosp Jt Dis. 2009;67(3):352-357. [12] MUJEEB A, MILLER AF, SAIANI A, et al. Self-assembled octapeptide scaffolds for in vitro chondrocyte culture. Acta Biomater. 2013;9(1): 4609-4617. [13] ALAVI A, STUPACK DG. Cell survival in a three-dimensional matrix. Methods Enzymol. 2007;426:85-101. [14] 郑欣欣,曹贵方.浅谈动物细胞体外三维培养技术[J].内蒙古科技与经济,2015(19):89-90. [15] 张振,罗辉宇,曾炼.大鼠软骨细胞与兔软骨细胞的培养与比较[J].骨科,2020,11(4):323-328. [16] 张辉,王少华,王茜,等. RGD多肽修饰多孔钽可激活MG63细胞整合素/黏着斑激酶信号通路[J].中国组织工程研究,2021,25(16): 2535-2540. [17] 崔逸爽,赖振权,胡中岭,等. 骨形态发生蛋白7复合国产多孔钽对骨髓间充质干细胞向软骨分化及功能的影响[J].中国组织工程研究,2020,24(16):2478-2484. [18] SUN X, YIN H, WANG Y, et al.In Situ Articular Cartilage Regeneration through Endogenous Reparative Cell Homing using a Functional Bone Marrow-specific Scaffolding System. ACS Appl Mater Interfaces. 2018;10(45):38715-38728. [19] 吴昊,李朝旭.关节软骨损伤修复的治疗进展[J].世界最新医学信息文摘,2019,19(93):75-77. [20] 尤奇,段小军,杨柳,等.临床应用软骨组织工程技术修复关节软骨缺损的进展[J].实用骨科杂志,2018,24(1):52-55. [21] DI LUCA A, LONGONI A, CRISCENTI G, et al.Surface energy and stiffness discrete gradients in additive manufactured scaffolds for osteochondral regeneration. Biofabrication. 2016;8(1):015014. [22] YU X, QIAN G, CHEN S, et al. A tracheal scaffold of gelatin-chondroitin sulfate-hyaluronan-polyvinyl alcohol with orientated porous structure. Carbohydr Polym. 2017;159:20-28. [23] 张辉,李亮,王茜,等.骨形成蛋白-7对多孔钽/软骨细胞复合物分泌功能以及Col-Ⅱ, AGG和Sox9基因表达的影响[J].北京大学学报(医学版),2015,47(2):219-225. [24] WANG Q, ZHANG H, LI Q, et al. Biocompatibility and osteogenic properties of porous tantalum. Exp Ther Med. 2015;9(3):780-786. [25] MARDONES RM, REINHOLZ GG, FITZSIMMONS JS, et al. Development of a biologic prosthetic composite for cartilage repair. Tissue Eng. 2005; 11(9-10):1368-1378. [26] HEMSHEKHAR M, THUSHARA RM, CHANDRANAYAKA S, et al. Emerging roles of hyaluronic acid bioscaffolds in tissue engineering and regenerative medicine. Int J Biol Macromol. 2016;86:917-928. [27] ORYAN A, KAMALI A, MOSHIRI A, et al. Chemical crosslinking of biopolymeric scaffolds: Current knowledge and future directions of crosslinked engineered bone scaffolds. Int J Biol Macromol. 2018;107: 678-688. [28] JUHÁSZ ML, MARMUR ES. Temporal fossa defects: techniques for injecting hyaluronic acid filler and complications after hyaluronic acid filler injection. J Cosmet Dermatol. 2015;14(3):254-259. [29] SILVA CR, BABO PS, GULINO M, et al. Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration. Acta Biomater. 2018;77:155-171. [30] MARTÍNEZ-SANZ E, OSSIPOV DA, HILBORN J, et al. Bone reservoir: Injectable hyaluronic acid hydrogel for minimal invasive bone augmentation. J Control Release. 2011;152(2):232-240. [31] 沈雁,唐毅,李斯明,等.碱性成纤维细胞生长因子与透明质酸对培养兔软骨细胞的作用[J].中华创伤杂志,2000,16(6):337-339. [32] 黄建荣,李卫平,沈慧勇.胰岛素样生长因子I与透明质酸对人关节软骨细胞的作用[J].中华生物医学工程杂志,2008,14(3):180-184. [33] 拉希德,刘世清.透明质酸对体外培养大鼠退变关节软骨细胞的影响[J].武汉大学学报(医学版),2007,28(2):177-180. [34] 杜国辉,屈爱存,陈建英,等.透明质酸对关节软骨蛋白聚糖aggrecan的影响[J].中国生化药物杂志,2011(2):45-47. [35] 高宗强,郭雄,陈君长,等.透明质酸对体外培养大骨节病软骨细胞Ⅱ型胶原和聚集蛋白聚糖mRNA表达的影响[J].中国组织工程研究,2009,13(46):9061-9065. [36] MUJEEB A, MILLER AF, SAIANI A, et al. Self-assembled octapeptide scaffolds for in vitro chondrocyte culture. Acta Biomater. 2013;9(1): 4609-4617. [37] 曲鹏玮,亓建洪,韩运宁,等.力学刺激对体外保存软骨活力影响的实验研究[J].中国医药生物技术,2018,13(3):243-248. [38] FITZGERALD JB, JIN M, DEAN D, et al. Mechanical Compression of Cartilage Explants Induces Multiple Time-dependent Gene Expression Patterns and Involves Intracellular Calcium and Cyclic AMP. J Biol Chem. 2004;279(19):19502-19511. [39] SHIEH AC, ATHANASIOU KA. Dynamic compression of single cells. Osteoarthritis Cartilage. 2007;15(3):328-334. [40] WALDMAN SD, SPITERI CG, GRYNPAS MD, et al. Effect of Biomechanical Conditioning on Cartilaginous Tissue Formation in Vitr. J Bone Joint Surg Am. 2003;2(1):101-105. [41] CHEN C, TAMBE DT, DENG L, et al. Biomechanical properties and mechanobiology of the articular chondrocyte. Am J Physiol Cell Physiol. 2013;305(12):C1202-C1208. [42] TOYODA T, SEEDHOM BB, KIRKHAM J, et al. Upregulation of aggrecan and type II collagen mRNA expression in bovine chondrocytes by the application of hydrostatic pressure. Biorheology. 2003;40:79-85. [43] JEON JE, SCHROBBACK K, HUTMACHER DW, et al. Dynamic compression improves biosynthesis of human zonal chondrocytes from osteoarthritis patients. Osteoarthritis Cartilage. 2012;20(8):906-915. |
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